大肠癌组织中乙醛脱氢酶1和分化簇133的表达及其临床意义

 目的 研究乙醛脱氢酶1(aldehyde dehydrogenase 1,ALDH1)和分化簇133(cluster of differentiation 133,CD133)在大肠癌中的表达,探讨其与大肠癌发生、发展及预后的关系。方法 采用免疫组织化学方法检测60例大肠癌、25例大肠腺瘤和20例正常大肠黏膜组织中ALDH1和CD133的表达,并分析其与临床病理参数和预后的关系。结果 大肠癌组织中ALDH1及CD133的表达率(78.3%,75.0%)明显高于大肠腺瘤(54.5%,48.0%)和正常大肠黏膜组织(15.0%,10.0%),每两组之间比较差异有统计学意义 (P<0.05) 。ALDH1的表达与大肠癌的浸润深度、TNM分期、淋巴结转移呈正相关 (P<0.05) ,与分化程度呈负相关 (P<0.05)。CD133的表达与大肠癌的浸润深度、淋巴结转移、TNM分期呈正相关 (P<0.05)。ALDH1和CD133在大肠癌中的表达呈正相关 (P<0.05)。ALDH1及CD133表达阳性的患者,其5年生存率低于表达阴性者 (P<0.05)。结论 ALDH1及CD133表达升高与大肠癌的发生、发展显著相关,有可能作为判定大肠癌预后的指标之一。

Expression and significance of ALDH1 and CD133 in colorectal carcinoma

Objective To study the expressions of ALDH1 and CD133 in colorectal carcinoma and their correlations with the occurrence, development and prognosis of colorectal carcinoma.Methods The expressions of ALDH1 and CD133 in 60 cases of colorectal carcinoma, 25 cases of colorectal adenomas and 20 cases of normal colorectal tissues were detected using immunohistochemical methods, and their association with clinicopathological parameters and prognosis was analyzed.Results The positive rate of ALDH1 and CD133 in colorectal carcinoma (78.3%,75.0%) was significantly higher than that in colorectal adenomas (54.5%,48.0%) and normal colorectal tissues (15%,10%) (P<0.05). The expression of ALDH1 was positively correlated with the depth of invasion, TNM stage and lymph node metastasis (P>0.05), but negatively with the degree of differentiation (P>0.05) in colorectal carcinoma. The expression of CD133 in colorectal carcinoma was positively correlated with the depth of invasion, lymph node metastasis and TNM staging (P<0.05). There was a positive correlation between the expressions of ALDH1 and CD133 in colorectal carcinoma (P<0.05). The 5-year survival rate of ALDH1 and CD133 positive patients was significantly lower than that of negative patients (P<0.05).Conclusions The increased expressions of ALDH1 and CD133 are significantly related to the occurrence and development of colorectal carcinoma and may be used as an indicator of the prognosis of colorectal carcinoma.