槐定碱阳离子脂质体的制备及体外抗肿瘤活性的研究

目的制备槐定碱阳离子脂质体,并探讨其对肿瘤细胞的抑制作用。方法采用主动载药法制备槐定碱阳离子脂质体,并对其进行表征研究,采用MTS方法考察槐定碱阳离子脂质体对3种肿瘤细胞的抑制作用。结果制备得到的槐定碱阳离子脂质体呈类圆形,表面光滑,其平均粒径和聚分散指数分别为242.2 nm和0.180,表面电荷为+32.5 m V,其包封率和载药量分别为88.62%和5.97%。槐定碱阳离子脂质体对3种肿瘤细胞的IC50值均明显高于槐定碱,而空白阳离子脂质体对细胞并无明显的抑制作用。结论采用阳离子脂质体作为槐定碱的载体有利于将药物透过细胞膜,提高抗肿瘤作用,值得进行深入的系统研究。

Study on Preparation and in Vitro Antitumor Activity of Sophoridine Cationic Liposomes

OBJECTIVE To prepare sophoridine cationic liposome, and study its inhibitory effect on tumor cells. METHODS Active-loading method was used to prepare sophoridine liposomes, their characterization were investigated, futher, MTS method was used to evaluate the inhibitory effects of sophoridine cationic liposome on 3 tumor cells. RESULTS The sophoridine cationic liposomes which had been prepared were round with smooth surface, their average particle size, PDI, surface charge, encapsulation efficiency and drug loading amount was 242.2 nm, 0.180, +32.5 mV, 88.62% and 5.97% respectively. The IC50 of sophoridine cationic liposome on 3 tumor cells were obviously lower than sophoridine, and the blank cationic liposomes had no obvious inhibitory effect to cells. CONCLUSION Using cationic liposome as carrier for Sophoridine makes the drugs through the cell membrane easier, and improves the anti-tumor effect, is worth in-depth research in future.