Constituents of Laurus nobilis L. inhibit recombinant human lanosterol synthase |
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Authors: | Rie Tanaka Yuichi Sakano Ken Shimizu Masaaki Shibuya Yutaka Ebizuka Yukihiro Goda |
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Institution: | (1) National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan;(2) Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;(3) Present address: San-Ei Gen F.F.I., Inc., Osaka, Japan |
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Abstract: | Extracts from 37 kinds of foods and foodstuffs were tested for inhibitory activity against recombinant human lanosterol synthase. Among them, extracts from five samples showed significant inhibition. Potent activity (55%) was found in 95% ethanol extract of Laurus nobilis L. Therefore, large-scale methanol extraction of the plant was carried out, and the constituents were separated by partition and fractionation by silica gel chromatography and HPLC. Four flavonoids, kaemperol 3-O-2 ,4 -O-di-E-p-coumaroyl- -l-pyranorhamnoside] (1); 3,3 ,4 ,5,6,7,8-heptamethoxyflavone (2); 3 ,4 ,5,6,7,8-hexamethoxyflavone (nobiletin) (3); and 4 ,5,6,7,8-pentamethoxyflavone (tangeretin) (4); and six sesquiterpens, eremanthine (5), dehydrocostus lactone (6), costunolide (7), zaluzanin C (8), zaluzanin D (9) and reynosin (10) were isolated. Eremanthine (5) showed the most potent activity, 70% inhibition, at the concentration of 500 M. |
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Keywords: | Lanosterol synthase Cholesterol biosynthesis Laurus nobilis Flavonoiod Sesquiterpene Bay leaf |
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