替代对照品法测定新疆孕马尿中3种主要结合雌激素含量

目的 建立HPLC替代对照品法测定新疆孕马尿中3种主要结合雌激素的含量。方法 采用HPLC在不同条件下测定替代对照品雌酮硫酸钠对于马烯雌酮硫酸钠及17α-双氢马烯雌酮硫酸钠对照品的校正因子，利用校正因子和替代对照品雌酮硫酸钠测定孕马尿中3种主要结合雌激素的含量。结果 以替代对照品法测得的结果与常规含量测定方法结果一致。结论 在高效液相色谱仪上使用替代对照品法测定孕马尿中的3种主要结合雌激素的含量，此方法准确可靠，可节约检测成本。

Determination of Three Main Conjugated Estrogens in Pregnant Mare Urine of Xinjiang by Substitute Reference Substance

OBJECTIVE To establish a method for determination of three main conjugated estrogens in pregnant mare urine of Xinjiang by substitute reference substance by HPLC. METHODS Sodium estrone sulfate was selected as the substitute for reference substance of sodium equilin sulfate and sodium 17α-dihydroequilin sulfate. The relative correction factors（RCF） of sodium equilin sulfate and sodium 17α-dihydroequilin were measured by HPLC under different conditions. Assay of three main conjugated estrogens in pregnant mare urine of Xinjiang was determined by substitute reference and the relative correction factors. RESULTS The quantitative results of the new method were almost consistent with the results of conventional HPLC. CONCLUSION The substitute reference substance is adopted in HPLC determination of three main conjugated estrogens in pregnant mare urine of Xinjiang. The new method is economical and practical.