| 基于植株性别差异的天花粉多糖、蛋白质含量比较 |
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| 引用本文： | 张波,刘红燕,李佳,彭艳丽.基于植株性别差异的天花粉多糖、蛋白质含量比较[J].中国药师,2014(4):617-619. |
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| 作者姓名： | 张波 刘红燕 李佳 彭艳丽 |
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| 作者单位： | 山东中医药大学 济南 250355;山东中医药大学 济南 250355;山东中医药大学 济南 250355;山东中医药大学 济南 250355 |
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| 基金项目： | 山东省科技发展计划项目( 编号：2011BAI06B06)；“山东道地药材资源质量控制科技平台建设”项目( 编号：2008GG2NS02022)；山东省农业良种工程项目(编号：2009LZ01-03) |
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| 摘 要： | 目的：比较雌雄株栝楼根中多糖、蛋白质含量差异，为天花粉主要来源于雄株栝楼的根提供理论依据。方法：水提醇沉法提取可溶性多糖，硫酸蒽酮比色法检测多糖含量；丙酮分级沉淀法提取天花粉蛋白，考马斯亮蓝染色紫外比色法检测蛋白含量。结果：雌、雄株栝楼根中多糖含量分别为2．628％、3．237％；雌、雄株天花粉蛋白含量分别为0．193％、0．280％，雄株天花粉多糖、天花粉蛋白含量均高于雌株。结论：以多糖和蛋白质含量为指标，雄株根入药优于雌株。
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| 关 键 词： | 天花粉 雌雄株 多糖 天花粉蛋白 |
| 收稿时间： | 2014/1/15 0:00:00 |
| 修稿时间： | 2014/2/13 0:00:00 |
Content Comparison of Polysaccharide and Protein in Male and Female Trichosanthis Radix |
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| Zhang Bo,Liu Hongyan,Li Jia and Peng Yanli.Content Comparison of Polysaccharide and Protein in Male and Female Trichosanthis Radix[J].China Pharmacist,2014(4):617-619. |
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| Authors: | Zhang Bo Liu Hongyan Li Jia and Peng Yanli |
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| Institution: | Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China |
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| Abstract: | Objective： To compare the contents of polysaccharide and protein in male and female Trichosanthis Radix to provide reasonable explanation for that male plant was better. Methods： Polysaccharides in Trichosanthis Radix were obtained by water-extrac- tion and ethanol-precipitation method, and the content was determined by anthrone-sulfuric acid method. Tichosanthin （TCS） was ob- tained by hydrochloric acid extraction and acetone precipitation. Based on the light absorption of Coomassie brilliant blue G-250 com- bined with proteins, the content of TCS was detected. Results： The polysaccharides content in male and female Trichosanthis Radix was 2. 628% and 3. 237% , TCS content was 0. 193% and 0. 280% , respectively. Conclusion： The contents of polysaccharides and TCS in male plant are higher than those in female plant. The male plant is a better source for Trichosanthis Radix. |
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| Keywords: | Trichosanthis Radix Male and female plant Polysaccharide Tichosanthin （TCS） |
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