正交试验法优选延胡索及丹参的醇提工艺条件

目的：研究乙醇提取延胡索及丹参的最佳工艺，以及考察药材的粉碎度对有效成分的影响。方法：以乙醇浓度、加量、提取时间及提取次数为考察因素，采用L9（3-4）正交表设计进行正交试验，以延胡索乙素、丹酚酸B的含量为考察指标，优选最佳提取工艺。结果：最佳工艺为延胡索粉碎成最粗粉，6倍量75％乙醇，提取2次，每次1．5h。结论：该工艺简单、可行，可以用于大规模生产。

Optimization of Alcohol Extraction Technique for Rhizoma Corydalis and Salvia Miltiorrhiza by Orthogonal Test

Objective ： To optimize the ethanol extraction process of Rhizoma Corydalis and Salvia miltiorrhiza, and study the effect of comminution degree of the medicinal materials on the effective constituent. Methods： Using the alcohol concentration, alcohol vol- ume, extraction time and extraction times as the impacting factors,the content of tetrahydropalmatine and salvianolic acid as the indi- ces, the extraction process was optimized by orthogonal test of L9 （34）. Result,s： The best extraction conditions were as follows： Rhizo- ma Corydalis was crushed into coarse powder, the material / liquid ratio was 1：6 with 75 % ethanol as the extraction solvent, extracting twice with 1.5h each. Conclusion： The extraction method is appropriate and simple, and can be used in the industrial production.