广西地区汉族2型糖尿病患者脂联素基因多态性研究

目的：研究脂联素基因外显子2单核苷酸多态性（SNP）＋45T→G多态性与广西地区汉族2型糖尿病（T2DM）、肥胖及胰岛素抵抗（ IR）的关系。方法：运用聚合酶链反应-限制性片段长度多态性（ PCR-RFLP）的方法,对313例无亲缘关系的广西地区汉族人群的正常糖耐量（NGT95例）、糖耐量减低（IGT103例）和T2DM115例检测脂联素基因外显子2的多态性,并与代谢值进行相关性研究。结果：广西地区汉族人群中含脂联素基因外显子2的SNP＋45GG基因型的人群高密度脂蛋白（ HDL-C）水平低于TT、TG基因型的人群（P〈0.05）,HOMA-IR高组的GG基因型相对于TT基因型的OR为2.876（95%CI：1.141~7.248）,即GG基因型发生IR的相对危险度是TT基因型的2.876倍。结论：汉族人群中,脂联素基因SNPs＋45 TT基因型的HDL-C较GG基因型频率高；GG基因型较TT基因型人群易产生IR。但汉族人群SNPs＋45基因型与T2DM及肥胖无关。

Gene Polymorphism of Adiponectin in Han Nationality with Type 2 Diabetes Mellitus from Guangxi

Objective：To evaluate the relationship between single nucleotide polymorphism （SNP） ＋45T→G in adiponectin gene exon 2 and type 2 diabetes mellitus （T2DM）, obesity and insulin resistance （IR） in Han population in Guangxi. Methods：Polymer-ase chain reaction-restriction fragment length polymorphism （ PCR-RFLP） was performed to identify the T-G polymorphism of adiponec-tin （exon 2） in 313 unrelated Han people from Guangxi including 95 normal glucose tolerance,103 impaired glucose tolerance and 115 type 2 diabetes mellitus. Results：In Han people of Guangxi, the SNP ＋45 GG genotype in adiponectin gene had significantly lower HDL-C levels than TT and TG genotypes in Han people（P〈0. 05）. The subjects with the GG genotype at position 45 had a signifi-cantly increased risk of IR （odds ratio of 2. 87695%CI：1. 141-7. 248]） compared with those with the TT genotype at position 45. Conclusion：In Han people of Guangxi, the SNP ＋45 TT genotype in adiponectin gene has significantly higher HDL-C levels than GG genotype. The subjects with the GG genotype at position 45 have a significantly increased risk of IR compared with those with the TT genotype, while obesity and T2DM are independent on SNP＋45 of adiponectin gene.