七叶皂苷钠对沙土鼠脑缺血再灌注损伤ET和CGRP的影响

目的：观察七叶皂苷钠对沙土鼠脑缺血再灌注模型脑组织匀浆中内皮素（ ET）和降钙素基因相关肽（ CGRP）含量的影响。方法：采用结扎双侧颈总动脉缺血10 min再灌注2 h,建立沙土鼠脑缺血再灌注模型。七叶皂苷钠（10,20,40 mg·kg-1）术前3 d开始腹腔注射给药,qd,术后1 h给药1次。再灌注2 h后,放射免疫法测定脑组织ET和CGRP含量。结果：七叶皂苷钠各剂量组可降低脑组织ET水平至28.69~37.03 ng·L-1,与模型组比较有显著性差异（P〈0.05或0.01）,对脑组织CGRP水平则无明显影响（P〉0.05）。结论：七叶皂苷钠可能通过降低脑组织ET水平,实现对沙土鼠脑缺血再灌注损伤的保护作用。

Effects of Sodium Aescinate on ET and CGRP Contents in Gerbils with Cerebral Ischemia/reperfusion Injury

Objective：To investigate the effects of sodium aescinate on endothelin （ ET ） and calcitonin gene -related peptide （ CGRP） contents in the gerbils with cerebral ischemia/reperfusion injury （ CI/R） . Methods：The gerbil model of CI/R was prepared by bilateral common carotid arteries ligation for 10 min followed by 2-hour reperfusion. Sodium aescinate （i. p. , 10, 20 and 40 mg· kg-1 ） was administered once a day for 3 days before the operation and once every 1 h after the operation, respectively. The levels of ET and CGRP in brain tissue homogenate were determined by a radioimmunoassay method. Results：Sodium aescinate significantly in-hibited the level of ET （28. 69-37. 03 ng·L-1） in the brain tissue of gerbils with CI/R compared with the model group （P＆lt;0. 05 or P〈0. 01）, while showed no evident influence on CGRP （P〉0. 05）. Conclusion：Sodium aescinate has obvious protective effects a-gainst CI/R in gerbils, which may be due to its inhibitory action on ET levels in brain tissue.