HPLC法测定灵芝二维甲硫氨酸胶囊中甲硫氨酸、维生素B1及维生素B2含量

目的：建立用HPLC法同时测定灵芝二维甲硫氨酸胶囊中甲硫氨酸、维生素B1及维生素B2含量的方法。方法：采用Ultimate？ AQ-C18色谱柱（250 mm ×4.6 mm,5μm,Welch公司）,流动相：甲醇-0.07 mol·L-1庚烷磺酸钠溶液（加14 ml三乙胺并加水至1000 ml,用磷酸调节pH至3.5）,线性梯度洗脱,检测波长为220 nm,流速：1.0 ml·min-1。结果：甲硫氨酸、维生素B1、维生素B2分别在0.5270~1.5100 mg·ml-1（r=0.9999）、0.04552~0.13656 mg·ml-1（r=0.9999）、0.01010~0.06058 mg·ml-1（r =0.9999）范围内线性关系良好,平均回收率分别为100.5%、97.7%、101.5%,RSD 分别为0.5%、1.0%、1.4%（n=9）。结论：本法操作简便、结果准确、可靠,可用于灵芝二维甲硫氨酸胶囊中甲硫氨酸、维生素B1、维生素B2的含量测定。

Determination of Methionine,Vitamin B1 and Vitamin B2 in Lingzhi Erwei Methionine Capsules by HPLC

Objective：To establish an HPLC method for the simultaneous determination of methionine, vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules. Methods：The determination was performed on a Ultimate？ AQ-C18 column（250 mm × 4. 6 mm, 5 μm,Welch Inc. ） with the detection wavelength at 220 nm. The mobile phase was composed of methanol and 0. 07 mol·L-1 sodium heptanesulfonate solution （14 ml triethylamine diluted with water to1 000ml, adjusting pH to 3. 5 with diluted orthophosphoric acid） with gradient elution, and the flow rate was 1. 0 ml·min-1. Results： Methionine, vitamin B1 and vitamin B2 was in good linearity within the range of 0.527 0-1.510 0 mg·ml-1 - （r =0.999 9）, 0.045 52-0.136 56 mg·ml-1 （r =0.999 9） and 0.010 10-0. 060 58 mg·ml-1（r=0. 999 9）, respectively. The mean recovery was 100. 5%, 97. 7% and 101. 5% with RSD of 0. 5%, 1. 0%and 1. 4%（n=9） accordingly. Conclusion：The method is simple, accurate, reliable and appropriate in the simultaneous determina-tion of methionine,vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules.