盐酸小檗碱脂质体的制备及其含量、包封率的测定

目的：制备盐酸小檗碱脂质体,并测定其包封率和脂质体各成分含量。方法：采用硫酸铵梯度法制备盐酸小檗碱脂质体,以超速离心法、微柱法、超滤法对盐酸小檗碱脂质体包封率的测定方法进行研究,以HPLC-ELSD测定脂质体各成分含量。结果：超速离心法能将未包封药物与脂质体很好地分离,最佳超速离心条件：离心速度为60000 r·min-1,离心时间为1 h,离心温度为10℃,脂质浓度为6 mg·ml-1。结论：包封率测定方法具有简单、快速分离等优点。 HPLC-ELSD能够同时测定脂质体各成分含量。

Preparation and Determination of Encapsulation Efficiency and Content of Bererine Hydrochloride Liposomes

Objective：To prepare bererine hydrochloride （ BER） liposomes and establish an effective method for the determination of content and entrapment efficiency. Methods：BER liposomes were prepared by ammonium sulfate gradient method. The encapsula-tion efficiency of BER liposomes was respectively determined by supercentrifugation method, microcolumn gel method and ultrafiltration method, and the content of every component in BER liposomes was detected by HPLC-ELSD. Results：The results showed that super-centrifugation method could precisely separate the free drug from the liposomes. The optimum parameters of supercentrifugation method were the centrifugal speed of 60 000 r·min-1 , the centrifugal time of 1 h, the centrifugal temperature of 10℃ and the lipid concentra-tion of 6 mg·ml-1 . Conclusion：The method is simple and sensitive with good separation efficiency. HPLC-ELSD can be used to de-termine the content of every component in BER liposomes.