首页 | 本学科首页   官方微博 | 高级检索  
     

H2O2对大鼠骨骼肌卫星细胞凋亡和线粒体膜电位的影响及EPO的保护作用
引用本文:罗林杰,李江华,董少红,陈科奇. H2O2对大鼠骨骼肌卫星细胞凋亡和线粒体膜电位的影响及EPO的保护作用[J]. 中国病理生理杂志, 2010, 26(2): 357-361. DOI: 1000-4718
作者姓名:罗林杰  李江华  董少红  陈科奇
作者单位:暨南大学第二临床学院,深圳市人民医院心内科,广东 深圳 518020
基金项目:深圳市科技局资助项目 
摘    要:目的: 探讨过氧化氢(H2O2)对大鼠骨骼肌卫星细胞(SMSC)凋亡和线粒体膜电位(MMP)的影响以及促红细胞生成素(EPO)的保护作用。方法: 取体外培养的骨骼肌卫星细胞,随机分为正常对照组、H2O2组、H2O2+EPO组,采用流式细胞仪(FCM)检测细胞凋亡率和MMP的平均荧光强度,Hoechst 33258染色后荧光显微镜观察凋亡细胞的形态学变化。结果: H2O2组凋亡发生率最高,为(22.13±1.79)%,经10、20、40 kU/L浓度的EPO预处理后凋亡率分别为(16.47±2.53)%、(4.97±0.55)%、(2.93±0.47)%;H2O2干预组MMP最低,为9.70±0.09,经10、20、40 kU/L浓度的EPO预处理后MMP分别为12.67±0.32、27.90±0.66、44.53±0.93,对照组凋亡率为(1.93±0.57)%,MMP为51.37±0.64;在H2O2干预组和低剂量EPO预干预组,Hoechst 33258染色呈现明显的凋亡征象。结论: EPO可抑制H2O2诱导的细胞凋亡并稳定线粒体膜电位。

关 键 词:过氧化氢  骨骼肌卫星细胞  细胞凋亡  线粒体膜电位  促红细胞生成素  
收稿时间:2009-05-26
修稿时间:2009-10-23

Effects of H_2O_2 on apoptosis of skeletal muscle satellite cell and mitochondrial membrane potential
LUO Lin-jie,LI Jiang-hua,DONG Shao-hong,CHEN Ke-qi. Effects of H_2O_2 on apoptosis of skeletal muscle satellite cell and mitochondrial membrane potential[J]. Chinese Journal of Pathophysiology, 2010, 26(2): 357-361. DOI: 1000-4718
Authors:LUO Lin-jie  LI Jiang-hua  DONG Shao-hong  CHEN Ke-qi
Affiliation:The Second Clinical College of Jinan University, Department of Cardiology, Shenzhen People’s Hospital, Shenzhen 528020, China. E-mail:luosj@tom.com
Abstract:AIM: To observe the effects of H2O2 on apoptosis of skeletal muscle satellite cells (SMSC)and mitochondrial membrane potential (MMP), and protective effect of erythropoietin(EPO). METHODS: SMSC in vitro were divided into three groups: H2O2 group, H2O2+EPO group and control. Apoptosis rate and the means were obverted by monofluorescence flow cytometry. The morphological change of apoptosis cells were observed under fluorescence microscopy after Hoechst 33258 staining. RESULTS: The cells in H2O2 group show the highest apoptosis rate (22.13±1.79)%. In H2O2+EPO group, apoptosis rate were (16.47±2.53)%, (4.97±0.55)% and (2.93±0.47)% according to the EPO treated levels (10, 20 or 40 kU/L), respectively. MMP level in H2O2 group was the lowest 9.70±0.09. MMP levels in H2O2+EPO group were 12.67±0.32, 27.90±0.66, 44.53±0.93, respectively according to the EPO treated levels (10, 20 or 40 kU/L). In control group, apoptosis rate was 1.93±0.57 and MMP was 51.37±0.64. In H2O2 group and H2O2+ low dosage EPO group, Hoechst 33258 staining showed obvious apoptosis. CONCLUSION: EPO inhibits the apoptosis induced by H2O2 and stabilizes the MMP, which is related to the dosage of EPO.
Keywords:Hydrogen peroxide  Skeletal muscle satellite cells  Apoptosis  Mitochondrial membrane potential  Erythropoietin
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国病理生理杂志》浏览原始摘要信息
点击此处可从《中国病理生理杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号