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| 茅莓及其含药血清对K562白血病细胞抑制作用的实验研究 | |
| 引用本文： | 许晓峰,张学进,冯健,姜铁军,付天红,高瑞兰. 茅莓及其含药血清对K562白血病细胞抑制作用的实验研究[J]. 中国中医药科技, 2011, 18(5): 408-410,368 |
| 作者姓名： | 许晓峰 张学进 冯健 姜铁军 付天红 高瑞兰 |
| 作者单位： | 1. 浙江省中西医结合医院·杭州,3100032 2. 浙江中医药大学·杭州,310053 3. 浙江省中医院·杭州,310006 |
| 基金项目： | 浙江省中医药管理局课题No.2008CB063 |
| 摘 要： | 目的：观察茅莓及其含药血清体内及体外对K562细胞的抑制增殖作用。方法：通过建立荷白血病瘤裸鼠模型，观察不同浓度的茅莓水煎液对荷白血病瘤裸鼠瘤体体积、重量、组织病理学改变的影响；通过半固体琼脂培养观察不同浓度的茅莓含药血清对K562细胞集落生长的抑制作用；通过MTT法观察不同浓度的含药血清对K562白血病细胞的抑制增殖作用。结果：茅莓水煎液高、中、低剂量组瘤体体积、瘤重均较模型对照组明显减少；茅莓水煎液组及阿糖胞苷（Ara—c）组光镜下可见多个坏死灶，瘤细胞肿胀、破裂，细胞核溶解，消失等现象。终浓度为10％和20％的含药血清做琼脂半固体集落培养，显示随着浓度增加，K562细胞集落形成明显减少，抑制作用呈刺量依赖性，MTT法显示终浓度10％和20％含药血清能明显抑制K562细胞增殖。结论：茅莓在荷白血病瘤裸鼠体内具有显著抑制瘤体生长的作用，在一定浓度下，其含药血清对K562细胞也有明显的抑制增殖作用。 |
| 关 键 词： | 白血病／中医药疗法 茅莓／治疗应用 血清药理学 裸鼠 大鼠 |
EXPERIMENTAL STUDY ON INHIBITION EFFECTS OF JAPANESE RASPBERREY HERB AND ITS SERUM CONTAINING HERB ON K562 LEUKEMIA CELLS |
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| XU Xiaofeng,ZHANG Xuejin,FENG Jian. EXPERIMENTAL STUDY ON INHIBITION EFFECTS OF JAPANESE RASPBERREY HERB AND ITS SERUM CONTAINING HERB ON K562 LEUKEMIA CELLS[J]. Chinese Journal of Traditional Medical Science and Technology, 2011, 18(5): 408-410,368 | |
| Authors: | XU Xiaofeng ZHANG Xuejin FENG Jian |
| Affiliation: | XU Xiaofeng,ZHANG Xuejin,FENG Jian(Hospital of Integrated Chinese Traditional and Western Medicine of Zhejiang Province,Hangzhou 310003) |
| Abstract: | Objective:To observe the inhibition of Japanese raspberry herb and its serum containing herb on proliferation of K562 leukemia cells in vivo and vitro.Methods:Models of K562-bearing mice were built by subcutaneous vaccination K562 leukemia cells,mice were divided into model,positive and raspberry low,middle,high groups,each group was given corresponding medicine after vaccination 5 days,observing the changes of tumor volume,weight and histopathology in each group mice after treated 14days.Observing the inhi... |
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