| 鸭乙型肝炎病毒YMDD突变株体外耐药模型建立 |
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| 引用本文： | 付喜花,梁蔚芳,吴小东,沈国俊,何海棠,陈金军,侯金林.鸭乙型肝炎病毒YMDD突变株体外耐药模型建立[J].南方医科大学学报,2011,31(4):633-636. |
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| 作者姓名： | 付喜花 梁蔚芳 吴小东 沈国俊 何海棠 陈金军 侯金林 |
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| 作者单位： | 南方医科大学南方医院感染内科;衡阳市中心医院消化内科; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)(2007CBS12901)~~ |
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| 摘 要： |
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| 关 键 词： | 鸭乙型肝炎病毒 定点突变 鸡肝癌细胞系 拉米夫定 |
Construction of a duck hepatitis B virus YMDD mutant and identification of its resistance phenotype |
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| FU Xi-hua,LIANG Wei-fang,WU Xiao-dong,SHEN Guo-jun,HE Hai-tang,CHEN Jin-jun,HOU Jin-lin.Construction of a duck hepatitis B virus YMDD mutant and identification of its resistance phenotype[J].Journal of Southern Medical University,2011,31(4):633-636. |
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| Authors: | FU Xi-hua LIANG Wei-fang WU Xiao-dong SHEN Guo-jun HE Hai-tang CHEN Jin-jun HOU Jin-lin |
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| Institution: | FU Xi-hua1,LIANG Wei-fang1,WU Xiao-dong2,SHEN Guo-jun1,HE Hai-tang1,CHEN Jin-jun1,HOU Jin-lin1 1Department of Infectious Diseases,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China,2Department of Gastroenterology,Hengyang Central Hospital,Hengyang 421001 |
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| Abstract: | Objective To construct a lamivudine-resistant plasmid containing 1.2 unit genome of duck hepatitis B virus and identify its replication and drug-resistance in avian LMH hepatica cells.Methods The recombinant plasmid PBS-DHBV1.2 was constructed using the 1.2-genome length DHBV DNA sequence from a dimer DHBV genome with pcDNA3.1 as the template.With site-directed mutagenesis,we obtained PBS-DHBV1.2-M512V plasmids with polymerase gene mutation from PBS-DHBV1.2.Two constructed plasmids were transiently transfec... |
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| Keywords: | duck hepatitis B virus site-directed mutagenesis LMH cells lamivudine |
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