|
|||||
|
|
Increased levels of microparticles originating from endothelial cells, platelets and erythrocytes in subjects with metabolic syndrome: Relationship with oxidative stress |
|
| Authors: | O. HelalC. Defoort,S. RobertC. Marin,N. LesavreJ. Lopez-Miranda,U. Risé rusS. Basu,J. LovegroveJ. McMonagle,H.M. RocheF. Dignat-George,D. Lairon |
| Affiliation: | a UMR INSERM 476, INRA 1260, Univ Aix-Marseille, Faculté de Médecine, Marseille F-13385, France b INSERM UMR 608, Laboratory of Immunology and Hematology, Faculté de Pharmacie, Université de la Méditerranée, CHU La Conception, Marseille, France c Hospital Universitario Reina Sofia, Unidad de Lipidos y arteriosclerosis, Universidad de Cordoba, CIBER de Fisiopatologia Obesidad y Nutricion (CB06/03), Instituto Salud CarlosIII, Cordoba, Spain d Assistance Publique Hopitaux Marseille, CIC, Marseille F-13009, France e Department of Public Health and Caring Sciences/Clinical Nutrition and Metabolism, Uppsala University, Uppsala 75185, Sweden f Department of Public Health and Caring Sciences/Oxidative stress and Inflammation, and Center of Excellence-Inflammation, Uppsala University Hospital, Uppsala 75185, Sweden g School of Food and Nutritional Sciences and Institute for Cardiovascular and Metabolic Research, University of Reading, RG6 6AP, UK h UCD Conway Institute, School of Public Health and Population Science, University College Dublin, Belfield, Dublin 4, Ireland |
| Abstract: | Background and aimsThe Metabolic Syndrome (MetS) is associated with increased cardiovascular risk. Circulating microparticles (MP) are involved in the pathogenesis of atherothrombotic disorders and are raised in individual with CVD. We measured their level and cellular origin in subjects with MetS and analyzed their associations with 1/anthropometric and biological parameters of MetS, 2/inflammation and oxidative stress markers.Methods and resultsEighty-eight subjects with the MetS according to the NCEP-ATPIII definition were enrolled in a bicentric study and compared to 27 healthy controls. AnnexinV-positive MP (TMP), MP derived from platelets (PMP), erythrocytes (ErMP), endothelial cells (EMP), leukocytes (LMP) and granulocytes (PNMP) were determined by flow cytometry. MetS subjects had significantly higher counts/μl of TMP (730.6 ± 49.7 vs 352.8 ± 35.6), PMP (416.0 ± 43.8 vs 250.5 ± 23.5), ErMP (243.8 ± 22.1 vs 73.6 ± 19.6) and EMP (7.8 ± 0.8 vs 4.0 ± 1.0) compared with controls. LMP and PNMP were not statistically different between groups. Multivariate analysis demonstrated that each criterion for the MetS influenced the number of TMP. Waist girth was a significant determinant of PMP and EMP level and blood pressure was correlated with EMP level. Glycemia positively correlated with PMP level whereas dyslipidemia influenced EMP and ErMP levels. Interestingly, the oxidative stress markers, plasma glutathione peroxydase and urinary 8-iso-prostaglandin F2 α, independently influenced TMP and PMP levels whereas inflammatory markers did not, irrespective of MP type.ConclusionIncreased levels of TMP, PMP, ErMP and EMP are associated with individual metabolic abnormalities of MetS and oxidative stress. Whether MP assessment may represent a marker for risk stratification or a target for pharmacological intervention deserves further investigation. |
| Keywords: | Platelets microparticles Endothelial microparticles Erythrocytes microparticles Metabolic syndrome Glutathione peroxydase 8-Iso-prostaglandin F2 α |
| 本文献已被 ScienceDirect 等数据库收录！ | |