骨良恶性肿瘤Bcl-2和p53蛋白表达及意义

【目的】探讨Ｂｃｌ－２、ｐ５３在良恶性骨肿瘤组织中的蛋白表达情况及其生物学意义。【方法】应用ＡＢＣ免疫组化方法对３３例恶性骨肿瘤，包括１９例骨肉瘤，１４例软骨肉瘤和２２例良性骨肿瘤（骨软骨瘤）组织中Ｂｃｌ－２，ｐ５３的蛋白表达进行研究。【结果】恶性骨肿瘤组织中Ｂｃｌ－２，ｐ５３蛋白的阳性表达率分别为５１．５％和５４．５％，其中骨肉瘤组织为６３．２％和４７．４％，软骨肉瘤组织为３５．７％和６４．３％；良性骨肿瘤组织阳性表达率为１８．２％和２２．７％。恶性骨肿瘤组织Ｂｃｌ－２，ｐ５３蛋白阳性表达率明显高于良性骨肿瘤（Ｐ＜０．０５），骨肉瘤组织Ｂｃｌ－２蛋白阳性表达率明显高于骨软骨瘤（Ｐ＜０．０１），软骨肉瘤组织ｐ５３蛋白阳性率明显高于骨软骨瘤（Ｐ＜００５）。ＡＫＰ值增高组的恶性骨肿瘤组织ｐ５３蛋白阳性表达率（７５％）明显高于ＡＫＰ值正常值组（２０％，Ｐ＜０．０５）。【结论】Ｂｃｌ－２癌基因介导的凋亡紊乱和ｐ５３肿瘤抑制基因突变失活与恶性肿瘤的发生有关，Ｂｃｌ－２蛋白表达活性可能与骨肿瘤的恶性程度有关，ｐ５３肿瘤抑制基因突变失活是恶性肿瘤细胞成骨活性增强的因素之一。标记羊抗鼠ＩｇＧ和ＡＢＣ试剂，购自Ｓｉｇｍａ公

Expression of Bcl-2 and p53 Oncoproteins in Benign and Malignant Bone Tumors

To study the expression and biological significance of Bcl-2 and p53 oncoproteins in benign and malignant bone tumors. The expression of Bcl-2 and p53 oncoproteins were investigated in 33 cases of malignant bone tumors,including 19 cases of osteosarcoma and 14 cases of chondrosarcoma,and 22 cases of benign bone tumors (osteochondroma) by using Avidin-Biotin Complex (ABC) immunohistochemical method. The positive expression rates of Bcl-2 and p53 oncoproteins were 51.5% and 54.5% in malignant bone tumors (osteosarcoma:63.2% and 47.4%;chondrosarcoma: 35.7% and 64.3%),whereas those in benign bone tumors were 18.2% and 22.7% respectively.The positive expression rates of Bcl-2 and p53 oncoproteins were significantly higher in malignant bone tumors than those in benign ones(P<0.05).The expression rate of Bcl-2 oncoprotein in osteosarcomas showed a positive rate significantly higher than that in osteochondromas (P<0.01),while the positive expression rate of p53 oncoprotein in chondrosarcomas was significantly higher than that in osteochondromas(P<0.05).The positive expression rate of p53 oncoprotein in the malignant bone tumors with increased AKP activities was distinctly higher than that with normal alkaline phosphatase(AKP) activities (75% vs 20%;P<0.05).ConclusionsThe genesis of malignant bone tumors may be related to the apoptosis disorders induced by Bcl-2 oncogene,and to the mutational inactivation of p53 tumor suppression gene.The degree of malignancy in bone tumors may be related to the expression efficiency of Bcl-2 oncoproteins.The mutational inactivation of p53 tumor suppression gene is one of the factors that promotes the osteogenic activity in malignant tumor cells of bone.