高效液相色谱柱后衍生法测定豚鼠鼻粘膜中组胺

通过比较各种分析柱的分离效果 ,建立用离子交换柱分离 ,邻苯二甲醛 (OPA)柱后衍生荧光检测并分析豚鼠鼻粘膜组织中组胺的液相色谱方法 ,并对流动相的离子强度和pH进行了优化 ,确定以 40mmol·L- 1 pH 5 .5 0的柠檬酸钠缓冲液作为流动相为最佳条件。该方法的最低检测限为 5 0nmol·L- 1 ,回收率 >92 % ,线性范围为 5 0nmol·L- 1～ 5 0 0 μmol·L- 1 ,组胺的保留时间为 13min 12s,组胺峰面积的相对标准差 <3%。

High-performance liquid chromatography determination of histamine in nasal mucosas of guinea pig after post-column derivatization

A rapid and simple method for determining histamine by postcolumn derivatization liquid chromatography with fluorescent detection was described. SCX weakly acidic cation exchange column was used as an analytical column. Histamine was eluted at 13?min 12?s by 40 mmol·L -1 of trisodium citrate (pH5.50) at flow rate of 1.0 ml·min -1 . The recoveries of histamine ranged from 1.0 μmol·L -1 to 100 μmol·L -1 were above 92%. The detection limit for histamine was 50 nmol·L -1 and the linear range for histamine was 50 nmol·L -1 ～500 μmol·L -1 .