大肠癌手术治疗前后血清蛋白质谱的变化

目的观察大肠癌手术前后血清蛋白质谱的变化,从而筛选特异性蛋白标志物。方法选用IMAC#3蛋白质芯片和SELDITOF蛋白质芯片技术,对64例大肠癌患者和40名正常人的血清蛋白质谱进行分析。结果通过对大肠癌术前血清与正常人血清蛋白质谱分析发现共有19个蛋白质表达量有明显差异。并获得分子量为5908.55Da等5个蛋白质组成的模板,可将大肠癌与正常人正确分组,其正确分组率分别为97.5%(56/64)和80.0%(32/40)。术后血清蛋白质谱中,原高表达的蛋白质明显下调。利用该模板诊断大肠癌的灵敏度达96.8%,特异性达92.5%。结论血清中可以筛选到诊断大肠癌的特异性蛋白标志物并用以预后的判断。SELDITOF蛋白质芯片技术为建立蛋白质模板用以早期诊断大肠癌提供了可靠的技术平台。

Analysis of the changes of serum proteomic spectra in the patients with colorectal cancer before and after surgical operation

Objective To study the changes of serum proteomic spectra in patients with colorectal cancer before and after surgical operation in order to detect the specific protein markers.Methods Proteomic spectra of 64 serum samples from patients with colorectal cancer before and after operation and 40 from normal individuals were generated by IMAC Cu proteinchip array and SELDI TOF mass spectroscopy.Results Nineteen differentially expressed proteins in serum were screened by analysis of protemic spectra of preoperative patients and normal individuals.We obtained 5 proteins (5 908.55 Da and so on) making up markers pattern which was able to class the patient team and normal team.Corresponding correct ratio was 97.5% (56/64) and 80.0% (32/40) respectively.The proteins that overexprssed in serum of preoperative patients were obviously down regulated.These markers pattern yieded 96.8% sensitivity and 92.5% specificity respectively.Conclusion Clssification system will provide a highly accurate and innovative approach for the early diagnosis of colorectal cancer and judgment of prognosis.SELDI TOF mass spectrometry is a useful tool for the detection and idenification of new protein markers in serum.