串珠素对大鼠平滑肌细胞增殖的影响及其机制的研究

目的 观察串珠素对于大鼠血管平滑肌细胞（vascular smooth muscle cells。VSMCs）增殖反应的影响以及细胞信号转导机制。方法以^3H-TdR掺人法测定VSMCs增殖。Westem blot方法检测FAK表达。结果 Perlecan加强碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）引起的VSMCs增殖反应，并诱导粘着斑激酶（focal adhesion kinase，FAK）表达上调。Perlecan抗体消除Perlecan促进bFGF致VSMCs增殖反应，并抑制FAK表达。结论 串珠素增强bFGF致大鼠平滑肌细胞的增殖反应，其机制涉及FAK表达上调。

Effect of Perlecan on Vascular Smooth Muscle Cells Proliferation and Mechanism

Objective To investigate the effect of perlecan on enhanced vascular smooth muscle cells proliferation and the signaling transduction mechanisms. Methods VSMCs culture in vitro and 3H-TdR incorporation was estimated as proliferation of VSMCs; The expression of FAK was detected by Western blot. Results Perlecan enhanced the proliferation of VSMCs stimulated by bFGF and up- regulated FAK expression(P<0.05). After preconditioned antibody of perlecan, the function that perlecan enhanced the proliferation of VSMCs stimulated by bFGF was abolished, and FAK was inhibited (P<0.05). Conclusion Perlecan can enhance the proliferation of VSMCs stimulated by bFGF. This function was related to up- regulation of FAK.