大鼠胸部撞击致肺损伤模型的建立

目的 建立一种胸部撞击致肺挫伤的大鼠模型.方法 将84只SD大鼠随机分成7组(n=12),采用自制的胸部撞击装置,用不同撞击能量(2.7、2.0、1.8、1.5、1.2、0.9和0 J)对大鼠胸部进行撞击造成双侧肺损伤.监测撞击前后大鼠循环和呼吸功能变化.撞击120 min后处死大鼠,开胸行创伤评分,观察心肺组织形态学变化,并进行病理学评分,计算肺组织湿干比和肺泡灌洗液蛋白含量.结果 用1.2J能量撞击大鼠胸部可致理想的双侧肺损伤而无明显心脏损伤,动物死亡率较低.结论 采用自制胸部撞击装置建立的大鼠双侧肺损伤模型可以较好地模拟胸部撞击后双侧肺挫伤伤情,可用1.2J的撞击能量进行后续实验研究.

A rat model for lung injury induced by chest impact trauma

Objective To establish a rat model for lung injury induced by chest impact trauma. Methods Eight-four healthy male Sprague-Dawley rats were randomly divided into 7 groups (n=12) and were subjected to different chest impact energies (2.7,2.0,1.8,1.5,1.2,0.9 and 0 J) to induce bilateral lung injury using a self-designed device. The circulatory and respiratory functions were monitored before and after injury. The rats were sacrificed 120 min after chest impact. The morphological changes of the hearts and lungs were observed and graded pathologically. The wet/dry mass ratios of lungs and the protein contents in bronchoalveolar lavage fluid were determined. Results It was found that rats in the 1.2 J group developed bilateral lung contusion without substantial cardiac muscle trauma, and the animals had a low mortality. Conclusion Our self-designed device can satisfactorily produce lung injury model induced by chest impact trauma, and 1.2 J might be an ideal option for following studies.