Evaluation of transferability of R-plasmid in bacteriocin-producing donors to bacteriocin-resistant recipients |
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Authors: | Usui Masaru Hiki Mototaka Murakami Koichi Ozawa Manao Nagai Hidetaka Asai Tetsuo |
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Affiliation: | National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo 185-8511, Japan. |
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Abstract: | Bacteriocin-producing Escherichia coli (donors) rapidly kill conventional recipient E. coli DH5α in conjugation experiments. To evaluate plasmid transferability of bacteriocin-producing donors, we established 2 different bacteriocin-resistant mutants derived from E. coli DH5α and used them as recipients. When the bacteriocin-resistant mutants were used in conjugation experiments, the transconjugant recovery from 20 bacteriocin-producing donors increased from 5% (1/20) to 65% (13/20), and the transfer frequencies increased. These results showed that bacteriocins inhibited the transfer of the R-plasmid from bacteriocin-producing donors. Thus, application of bacteriocin-resistant recipients might aid the evaluation of the potential transferability of plasmids from bacteriocin-producing donors. |
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