白藜芦醇对大鼠肺纤维化逆转作用的研究

目的观察白黎芦醇(RES)对博来霉素(BLM)致大鼠肺纤维化的逆转作用。方法将60只SD大鼠分为对照组、模型组、RES中剂量干预组(50mg/kg)及RES高剂量干预组(100mg/kg),以气管内滴注BLM制造肺纤维化模型;于第14d起用RES进行干预,分别取第17d、24d及31d三个时间点对大鼠肺组织行MASSON染色、羟脯氨酸含量(HYP)测定及TGF-β1mRNA测定。结果模型组第17天,大鼠肺组织呈现典型肺纤维化,RES各干预组较模型组纤维化程度随时间变化而有所减轻;模型组中HYP含量(751.80±22.5、996.00±39.48、1197.60±105.98)明显高于对照组(266.00±24.08、307.00±16.40、318.00±18.7,P<0.05);RES高剂量干预组中各时间点HYP含量(657.45±18.31、514.34±9.78,504.20±13.16)均较模型组(751.80±22.52、996.00±39.48、1197.60±105.98)下降(P<0.05);模型组中TGF-β1mRNA含量在第17天(1.000±0.782)较对照组(0.490±0.039)高表达,使用RES高剂量干预后TGF-β1mRNA各时间点(0.788±0.205、0.472±0.199、0.474±0.310)较模型组(1.000±0.782、0.759±0.226、0.603±0.147)均降低(P<0.05)。结论 RES可能通过调节TGF-β1对已形成的大鼠肺纤维化有逆转作用。

Reverse of Bleomycin-induced Pulmonary Fibrosis of Rats by the Effects of Resveratrol

Objective To observe the reverse of bleomycin-induced pulmonary fibrosis of the rat by the effects of resveratrol.Methods Sixty male SD rats were randomly divided into control group,model group,and middle(50 mg/kg) ,high(100 mg/kg) resveratrol-treated groups.Rat pulmonary fibrosis was reproduced by an intratracheal injection of bleomycin to observe the inflammation and fibrosis in lung tissue changing,detect the hydroxyproline content in the lung tissues,and RT-PCR was used for determination of TGF-β1 mRNA in rats.Results The model group on the 17th day,in rat lung tissue,showed typical pulmonary fibrosis in the use of RES intervention group than in model group,the degree of fibrosis change reduced with time;In the model group(HYP content of 751.80 ± 22.5,996.00 ± 39.48,1 197.60 ± 105.98,P <0.05) was significantly higher than that of the control group(266.00 ± 24.08,307.00 ± 16.40,318.00 ± 18.7,P <0.05) ;High dose of RES in the intervention group at each time point HYP content(657.45 ± 18.31,514.34 ± 9.78,504.20 ± 13.16) were compared with the model group(751.80 ± 22.52,996.00 ± 39.48,1197.60 ± 105.98) decreased(P <0.05) ;The model group TGF-β1 mRNA content on the 17th day was highly expressed than the control group,the use of high dose of RES after the intervention of TGF beta1 mRNA all time points(0.788 ± 0.205,0.472 ± 0.199,0.474 ± 0.310) than those in the model group(1.000 ± 0.782,0.759 ± 0.226,0.603 ± 0.147) .Conclusion RES may reverse the pulmonary fibrosis of rats through the regulation of TGF-β1.