| 人胚胎干细胞建系及其研究进展 |
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| 引用本文： | 徐晨明,黄荷凤. 人胚胎干细胞建系及其研究进展[J]. 浙江大学学报(医学版), 2008, 37(1): 103-107. DOI: 10.3785/j.issn.1008-9292.2008.01.019 |
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| 作者姓名： | 徐晨明 黄荷凤 |
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| 作者单位： | 浙江大学医学院,附属妇产科医院,浙江,杭州,310006 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 浙江省重大专项基金 |
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| 摘 要： | 人胚胎干细胞(human embryonic stem cell,hES)在再生医学、药物筛选和发育生物学等领域具有重要的研究和应用价值。hES细胞系通常生长在小鼠胚胎成纤维细胞上,动物源性病原生物的污染风险限制了这类hES的临床应用,因此寻找无血清、无饲养层细胞的成分明确的培养体系进行hES细胞建系及培养,生产细胞移植临床安全应用标准的hES是近年hES建系的努力方向。
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| 关 键 词： | 干细胞/细胞学 胚胎/细胞学 细胞分化 细胞培养技术 细胞,培养的 人胚胎干细胞 研究进展 derivation Progress embryonic stem cells human 方向 应用标准 临床安全 细胞移植 生产 培养体系 成分 饲养层细胞 无血清 临床应用 污染风险 病原生物 动物源性 小鼠胚胎成纤维细胞 |
| 文章编号： | 1008-9292（2008）01-0103-05 |
| 收稿时间： | 2007-05-31 |
| 修稿时间： | 2007-07-17 |
Progress in the derivation,culture of human embryonic stem cells |
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| XU Chen-ming,HUANG He-feng. Progress in the derivation,culture of human embryonic stem cells[J]. Journal of Zhejiang University. Medical sciences, 2008, 37(1): 103-107. DOI: 10.3785/j.issn.1008-9292.2008.01.019 |
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| Authors: | XU Chen-ming HUANG He-feng |
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| Affiliation: | Department of Reproductive Endocrinology, The Obstetrics and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China. |
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| Abstract: | Human embryonic stem(hES) cells are considered to be a valuable resource for research in regenerative medicine,drug screening,and developmental studies.However,hES cells are usually established and maintained on mouse embryonic fibroblast feeder layers,and the risk of animal origin contamination from feeder layer generally excludes the clinical use of these hES cells.The main emphasis over the last several years has been in finding defined serum-and feeder layer-free system for derivation and culture of hES cells to enable the clinical use of hES cell for cell transplantation. |
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| Keywords: | Stem cells/cytol Embryo/cytol Cell differentiation Cell culture techniques Cells cultured |
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