半叶马尾藻多糖的分离纯化与光谱分析

本文对半叶马尾藻多糖采用水提醇沉法粗提，蛋白酶联合Sevag法去蛋白纯化，凝胶过滤柱层析分离得半叶马尾藻纯多糖的不同组分，溴化钾压片红外光谱测定和紫外光谱扫描鉴定分析。结果表明，SHP的苯酚-硫酸呈色反应呈阳性，茚三酮反应呈弱阳性，碘-碘化钾反应呈阴性，说明提取物为非淀粉性多糖。SHP的提取率为8．56％，总糖含量为90．96％。凝胶过滤柱层析结果表明，半叶马尾藻多糖中含有吡喃多糖、木聚糖、半乳糖、阿拉伯糖、葡萄糖、鼠李糖和果糖等。紫外扫描结果显示多糖几乎不含核酸和蛋白质；红外光谱显示SHP主要为吡喃多糖，并显示多糖分子结构中存在β2糖苷键，还存在α-D-半乳吡喃糖的特征吸收峰。

Separation, Purification and Spectrum Analysis of SHP

Crude SHP was obtained from extracting with hot water and precipitating with ethyl alcohol. Free proteins were removed by trypsinase combined with Sevage method. Different fractions were got by gel filtration chromatography. The molecule structure was analyzed by ultraviolet spectrum scanning and KBr infrared spectroscope. The results showed that the reaction of phenol-sulfuric acid was postiive, reaction of ninhydrin was weak positive and reaction of iodine-potassium iodide was negative, the extraction was regarded as a non-starch polysaccharide. Extraction rate of SHP was 8.56% and the content of polysaccharides was 90.96%. Gel filtration chromatography showed that SHP composed of furanopolysaccharides, xylose, galactose, arabinose, glucose, rhamnose and fructose. Ultraviolet spectrum showed that SHP contained little DNA and protein, infrared spectrum showed that SHP was main furanopolysaccharides and had beta-glycosidic bouds in its molecule structure, and there was a characteristic absorb peak of at-D-galactopyranosyl in it.