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| 多发性骨髓瘤分子细胞遗传学异常的研究 | |
| 引用本文： | 刘淑艳,李建勇,陈丽娟,黄金文,潘金兰,仇海荣,沈云峰,徐卫,薛永权. 多发性骨髓瘤分子细胞遗传学异常的研究[J]. 中华血液学杂志, 2007, 28(4): 223-226 |
| 作者姓名： | 刘淑艳 李建勇 陈丽娟 黄金文 潘金兰 仇海荣 沈云峰 徐卫 薛永权 |
| 作者单位： | 1. 浙江大学附属邵逸夫医院血液科,杭州310016 2. 210019,南京医科大学第一附属医院、江苏省人民医院血液科 3. 浙江大学附属邵逸夫医院血液科 4. 苏州大学附属第一医院、江苏省血液研究所 5. 南京医科大学附属无锡第一人民医院血液科 |
| 基金项目： | 江苏省社会发展基金资助项目（BS2006071）;江苏省“135工程”重点人才基金资助项目（RC2002044）;江苏省135工程重点学科开放课题项目（WK200210）. |
| 摘 要： | 目的探讨多发性骨髓瘤（MM）的分子细胞遗传学异常。方法应用CD138单克隆抗体磁珠分选系统纯化23例初治MM患者的骨髓浆细胞，结合一组探针和间期荧光原位杂交技术检测MM患者13q14缺失、p53缺失以及IgH基因重排的发生率。结果23例MM患者中，10例（43．5％）13q14缺失。阳性率为79％-96％；11例（47．8％）IgH基因重排；7例（30．4％）有13q14缺失和IgH基因重排；所有病例均未检测到p53基因缺失。结论13q14缺失及IgH基因重排在MM患者中的发生率较高；13q14的缺失和IgH基因重排的发生率同疾病进展、预后的关系有待进一步研究。 |
| 关 键 词： | 多发性骨髓瘤 浆细胞 原位杂交 荧光 遗传学研究 |
| 修稿时间： | 2006-08-21 |
Study on molecular cytogenetic abnormalities in multiple myeloma |
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| LIU Shu-yan,LI Jian-yong,CHEN Li-juan,HUANG Jin-wen,PAN Jin-lan,QIU Hai-rong,SHEN Yun-feng,XU Wei,XUE Yong-quan. Study on molecular cytogenetic abnormalities in multiple myeloma[J]. Chinese Journal of Hematology, 2007, 28(4): 223-226 | |
| Authors: | LIU Shu-yan LI Jian-yong CHEN Li-juan HUANG Jin-wen PAN Jin-lan QIU Hai-rong SHEN Yun-feng XU Wei XUE Yong-quan |
| Affiliation: | Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China |
| Abstract: | OBJECTIVE: To explore the molecular cytogenetic abnormalities in multiple myeloma (MM). METHODS: Bone marrow plasma cells from 23 previously untreated MM patients were purified by CD138 McAb magnetic cell sorting system, and a panel of probes for interphase fluorescence in situ hybridization were used to detect the 13q14 deletion, p53 deletion and IgH gene translocation in the sorted MM cells. RESULTS: Among 23 MM patients, 13q14 deletion was observed in 10 (43.5%) cases, with the positive rate of 13q14 deleted cells ranged from 79% to 96%; 14q32 translocation was observed in 11 (47.8%) cases; 13q14 deletion and 14q32 translocation were simultaneously observed in 7 (30.4%) cases; and p53 deletion was observed in none of the 23 cases. CONCLUSION: The frequency of 13q14 deletion and IgH gene translocation in multiple myeloma are high; and the relationship between 13q14 deletion, IgH gene translocation and prognosis is worth further investigating. |
| Keywords: | Multiple myeloma Plasma cells In situ hybridization, fluorescence Genetic research |
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