单克隆胶体金法与化学法测定隐血比较

目的:比较单克隆胶体金法与化学法测定粪便和胃引流物隐血。方法:将38例临床确诊为上消化道出血患者的"柏油"样粪便和16份经镜检证实含红细胞的胃引流物标本分别用胶体金试纸、邻联甲苯胺和四甲基联苯胺尿隐血试纸测试其是否含有隐血。结果:测试38例"柏油"样粪便标本,胶体金试纸阳性率为60.5%,邻联甲苯胺和尿隐血试纸均为100%;测试16份含红细胞的胃引流物标本,胶体金试纸阳性率为68.8%,邻联甲苯胺为100%,尿隐血试为81.3%。结论:高浓度血红蛋白以及血红蛋白被酸、碱化的标本和痰液等含大分子蛋白标本使胶体金法测定隐血的灵敏度下降,不适宜的pH可使该法特异性降低,而化学法所受影响较小。

Comparison of Monoclonal Colloidal Gold Method and Chemical Method in Detecting Occult Blood

Objective To compare the method determining the occult blood from stool and stomach strains by monoclonal colloidal gold method and chemical method.Methods Tarry stools from 38 cases definited upper gastrointestinal bleeding and 16 stomach strains confirmed including red blood cells by test under microscope respectively tested by colloidal gold indicator paper,ortho-tolidine method and tetramethyl benzidine indicator paper.Results In 38 cases of tarry stools,the positive percentage is 60.5 by colloidal gold indicator paper,and the positive percentage is100 byortho-tolidine method and tetramethyl benzidine indicator paper.In 16 cases of stomach strains the positive percentage is respectively 68.8,100 and 81.3 by above three methods.Conclusion Results show that thesensibility can be depressed by the method of colloidal gold method when the haematoglobin concentration is too high and haematoglobin is acidated or alkalified.Not suitable PH degree can depressed the specifity by the colloidal gold method,But chemical method is seldomly effected.