参附注射液对肝缺血再灌注大鼠血浆前列环素和血栓素A2及肝组织ATP酶的影响

目的:探讨参附注射液对大鼠肝缺血再灌注损伤的保护作用及其机制。方法:24只Wistar大鼠随机分为模型组和参附注射液(Shenfu Injection,SF)治疗组。SF治疗组大鼠腹腔注射参附注射液10ml/kg。模型组大鼠给予相同剂量的生理盐水。两组均采用Pringle's法阻断肝门缺血15min再灌注1h和3h,测定血浆血栓素B2(thromboxane B2,TXB2)和6-酮-前列腺素F1α(6-keto-prosta-glandin F1α,6-keto-PGF1α)以及肝组织匀浆Na -K -ATP酶、Ca2 -Mg2 -ATP酶的变化,并观察肝组织形态学改变。结果:再灌注3h SF治疗组血浆TXB2低于模型组,6-keto-PGF1α高于模型组,两者比值TXB2/6-keto-PGF1α低于模型组;再灌注1h、3h SF治疗组Na -K -ATP酶和Ca2 -Mg2 -ATP酶活性高于模型组。SF治疗组肝实质细胞和线粒体损伤明显减轻。结论:参附注射液对肝缺血再灌注损伤有保护作用,其机制与降低TXA2/PGI2比值,提高Na -K -ATP酶和Ca2 -Mg2 -ATP酶活性有关。

Effects of Shenfu Injection on prostacyclin, thromboxane A2 and activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury

OBJECTIVE: To explore the effects of Shenfu Injection on prostacyclin, thromboxane A2 and the activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury. METHODS: Twenty-four male Wistar rats weighing 200-250 g were randomly divided into two groups: Shenfu Injection (SF)-treated group (rats were treated with Shenfu Injection of 10 ml/kg through intraperitoneal injection) and untreated group (rats were administered with normal saline at the same dose and served as a control group). Hepatic ischemia was caused by Pringle's maneuver and lasted for fifteen minutes, and then one-hour or three-hour reperfusion was performed. Venous blood samples for the measurement of thromboxane B(2) (TXB(2)) and 6-keto-prostaglandin F(1 alpha)(6-keto-PGF(1 alpha)) were collected three hours after reperfusion. Liver tissue samples were collected one hour or three hours after reperfusion for the measurement of Na(+)-K(+)-ATPase and Ca(+)-Mg(+)-ATPase and for morphological studies. RESULTS: Plasma TXB(2) was lower in the SF-treated group than that in the untreated group after three-hour reperfusion (P>0.05), while 6-keto-PGF(1 alpha) was higher in the SF-treated group than that in the untreated group (P>0.05). The ratio of TXB(2) and 6-keto-PGF(1 alpha) was significantly lower in the SF-treated group than that in the untreated group (P<0.05). The activities of Na(+)-K(+)-ATPase and Ca(+)-Mg(+)-ATPase in the SF-treated group were improved obviously. A three-hour reperfusion after fifteen-minute ischemia caused important hepatic histological alterations. Marked structural abnormalities were observed in the untreated group, such as massive hepatocyte swelling, necrosis, mitochondria edema and vacuolar changes. In the SF-treated group, hepatic tissue injury was reduced significantly. CONCLUSION: Shenfu Injection protects hepatic tissue from ischemia-reperfusion injury, and such protective effects are achieved by decreasing the ratio of thromboxane A(2) and prostacyclin, and increasing the activities of Na(+)-K(+)-ATPase and Ca(+)-Mg(+)- ATPase.