| 玻璃体内注射庆大霉素致视网膜损伤的实验研究Ⅱ.组织化学定量分析 |
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| 引用本文： | 王丹梅,朱秀安,李凤鸣,李子良.玻璃体内注射庆大霉素致视网膜损伤的实验研究Ⅱ.组织化学定量分析[J].中华眼底病杂志,1994,10(4):232-235. |
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| 作者姓名： | 王丹梅 朱秀安 李凤鸣 李子良 |
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| 作者单位： | 北京医科大学附属第三医院眼科，江苏省徐州市眼病防治研究所 |
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| 基金项目： | 国家教委博士点基金，江苏省卫生厅科研课题基金 |
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| 摘 要： | 为探讨庆大霉素致视网膜损伤后视网膜组织内酶化学组份改变，采用微机分析组化定量方法，分析了注射5种不同剂量庆大霉素的兔视网膜内细胞色素氧化酶和乙酰胆碱酯酶改变，并通过超微结构观察进行比较。结果：100μg庆大霉素注射后3天即可引起细胞色素氧化酶流行性下降，随注入剂量增加或注射后观察时间延长，均呈逐渐下降趋势。100~500μg注射后3天乙酰胆碱酯酶活性变化轻微，7天后其活性受到明显抑制。1000~3000μg注射后3天两种酶活性即完全受到抑制。
（中华眼底病杂志，1994，10：232-235）
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| 关 键 词： | 庆大霉素，视网膜，中毒，细胞色素氧化酶，乙酰胆碱酯酶 |
| 收稿时间： | 1993-06-07 |
| 修稿时间： | 1994-04-22 |
RETINAL TOXICITY OF INTRAVITREAL GENTAMYCIN IN RABBITS Ⅱ.A HISTOCHEMICAL QUANTITATIVE ANALYSIS |
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| Wang Danmei,Zhu Xiuan,Li Fengming,et al..RETINAL TOXICITY OF INTRAVITREAL GENTAMYCIN IN RABBITS Ⅱ.A HISTOCHEMICAL QUANTITATIVE ANALYSIS[J].Chinese Journal of Ocular Fundus Diseases,1994,10(4):232-235. |
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| Authors: | Wang Danmei Zhu Xiuan Li Fengming |
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| Institution: | Dept. of Ophthalmology,Third Teaching Hospital,Beijing Medical University,100083 |
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| Abstract: | In order to explore the histochemical changes in retina after intravitreal injection of gentamycin,a histochemical quantitative analysis of cytochrome oxidase(CYO)and acetylcholinesterase(ACHE)was performed with a computerized image analysis system and was compared with that of morphological study.The results showed that CYO decreased significantly in 100μg dosage group.With increasing intravitreal gentamycin dosage or observed days,CYO decreased gradually in all rabbits.In 100~500μg dosage groups,ACHE changed mildly at 3 days of injection.It decreased significantly at 7 days.However,it was destroyed completely in 1000~3000μg dosage groups at 3 days.
（Chin J Ocul Fundus Dis,1994,10:232-235） |
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| Keywords: | gentamycin retina toxication cytochrome oxidase acetyl-cholinesterase |
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