小鼠骨髓程序移植的实验研究

目的：探讨进一步提高骨髓移植效果的新方法。方法：以昆明种小鼠急性放射病为模型，进行了骨髓程序移植后小鼠活存率及造血重建、急性移植物抗宿主病（ＧＶＨＤ）的观察。结果：骨髓程序移植可使小鼠移植细胞数降至１０５数量级，活存率达３０％；骨髓程序移植４×１０６个细胞时，于照射后１７天小鼠外周血白细胞、骨髓有核细胞计数显著回升，ＣＦＵＥ、ＣＦＵＧＭ、ＣＦＵＳ、ＣＦＵＦ已达正常，ＧＶＨＤ较一次移植１×１０７骨髓细胞组轻，活存率达６０％，显著高于一次骨髓移植组（３０％）。结论：骨髓程序移植可能充分利用了每次腾出的“龛位”，增加干细胞植入，却减少了移植细胞数，减轻了ＧＶＨＤ，提高了移植效果，是一种较好的移植新方法

Experimental study on mice scheduled bone marrow transplantation

OBJECTIVE: To enhance the grafting efficiency of bone marrow transplantation. METHODS: Lethally irradiated recipient Kunming mice were transplanted with bone marrow cells from normal Kunming mice either in one large (10(7)) number (BMT group) or in four separate small (10(5)-10(6)) numbers (scheduled BMT group) and the survival rate, hematopoiesis reconstitution and acute graft versus host disease (aGVHD) were compared between the two groups. RESULTS: Both BMT and SBMT (10(5) x 4) groups obtained the same survival rate of 30%. In SBMT (10(6) x 4) group, the peripheral WBC count, bone marrow nucleated cells and CFU-E, CFU-GM, CFU-S and CFU-F yields all returned to normal 17 days after irradiation. The degree of GVHD in SBMT group was less severe than that in BMT group, and the survival rate (60%) was significantly higher than that in BMT group (30%). CONCLUSION: SBMT can significantly enhance the grafting efficiency.