酒精性肝病大鼠创伤弧菌脓毒症凝血因子的动态变化

目的探讨酒精性肝病大鼠创伤弧菌脓毒症凝血因子的变化及意义。方法制作酒精性肝病大鼠创伤弧菌脓毒症模型,观察脓毒症组大鼠在染菌后2、6、12、24 h各时间点脓毒症表现,并检测各时间点大鼠血浆FIG、AT:A、tPA、PAI-1水平。结果脓毒症组大鼠染菌后6 h开始出现较明显的毒血症状,并随时间的延长而加剧,24 h达高峰,濒临死亡。脓毒症组大鼠血浆FIG在染菌后呈进行性升高,6 h始明显高于正常对照N组及酒精肝对照A5组(P<0.01),12 h达高峰,24 h FIG有下降趋势,但与12 h组相比,两者差异无统计学意义(P>0.05)。大鼠AT:A在染菌后呈进行性下降,6 h即显著低于A5及N组(P均<0.01)。tPA在染菌后2 h第1次达高峰(P<0.05),下降至正常水平后于24 h再次显著升高(P<0.01),PAI-1于2 h显著升高(P<0.01),而tPA/PAI值2 h显著降低,24 h显著高于对照组(P均<0.05)。结论酒精性肝病大鼠创伤弧菌脓毒症早期即可出现明显的凝血紊乱,动态检测FIG、AT:A、tPA、PAI-1水平可反映创伤弧菌脓毒症病情严重程度。

Dynamic changes of blood coagulation factors in rat model of alcoholic liver disease with Vibrio vulnificus sepsis

Objective To investigate the dynamic changes of blood coagulation factors in rat medol of alcoholic liver disease with vibrio vulnificus sepsis and try to find out its significance.Methods Based on vibrio vulnificus sepsis model in SD rats with alcoholic liver desease,the signs of sepsis,the levels of blood coagulation factors(including FIG,AT:A,tPA,PAI-1)were tested at the different time of 2 h,6 h,12 h and 24 h after innoculation with vibrio vulnificus.Results The apparente toxemic symptoms of sepsis began 6 h after innoculation,then the symptoms aggravated gradually and get the top at 24 h.Compared with the control groups(N and A6),the levels of FIG increased markedly at 6 h(P<0.01),and got the top at 12 h.However,it droped back slightly at 24 h(P>0.05,compared with group 12 h).The levels of AT:A decreased gradually and significantly in groups of 6 h,12 h,24 h.tPA in- creased markedly(P<0.05)in groups of 2 h and 24 h and PAI-1 increased markedly(P<0.01)at 2 h,whereas the ratio(tPA/PAI-1)decreased significantly(P<0.05)at 2 h,but increased markly(P<0.05)at 24 h compared with control groups.Conclusion Coagulopathy appears in early stage of vibrio vulnificus sepsis in rat model of alcoholic liver disease and the levels of FIG,AT:A,tPA,PAI-1 can in- dicate the severity of disease.