银屑病及几种皮肤肿瘤皮损中PI3K的表达

目的 探讨PI3K亚单位P85及P110与银屑病发病的相关性。方法 采用免疫组化法检测寻常性银屑病、慢性湿疹、脂溢性角化、皮肤鳞状细胞癌、基底细胞癌皮损和正常人皮肤中P85和P110的表达,对免疫染色的强度进行吸光度测定,统计学处理采用方差分析。结果 上述各组表皮中,P85的表达差异无统计学意义,FP85 = 0.21,P > 0.05;P110的表达差异有统计学意义,FP110 = 35.64,P < 0.01。与各组比较,银屑病皮损表皮P110的表达均明显升高,差异均有统计学意义,其余各组间差异无统计学意义（P > 0.05）。各组皮损的浸润淋巴细胞中P85和P110的表达差异均有统计学意义,FP85 = 59.98,P < 0.01;FP110 = 323.23,P < 0.01;鳞状细胞癌组及银屑病组分别与其余各组比较,差异均有统计学意义;银屑病与鳞状细胞癌组相比,差异无统计学意义（P > 0.05）。结论 P110表达的增强可能与角质形成细胞的增殖过速密切相关;而P110和P85的高表达是否与银屑病皮损内淋巴细胞的活化及增殖有关尚需进一步的研究。

Expression of phosphatidylinositol-3-kinase in the lesions of psoriasis and some skin tumors

Objective To investigate the relationship of the expression of phosphatidylinositol-3-kinase(PI3K)subunits,P85 and P110 to the pathogenesis of psoriasis.Methods Immunohistochemical staining for P85 and P110 was performed in the tissue specimens from patients with psoriasis(n=30),chronic dermatitis(n=20),seborrheic keratosis(n=20),squamous cell carcinoma(n=20),basal cell carcinoma(n=30)and normal human controls(n=10).The absorbance of immunostained tissue was quantified with image analysis system (Q550CW,Leica,Manheim,Germany).Statistical analysis was carried out by ANOVA,Results Among these groups,a significant difference was observed in the expression level of P110 in the epidermis(F=35.64,P＜0.01),as well as in that of P85(F=59.98,P＜0.01)and P110(F=323.23,P＜0.01)in the lymphocytes infiltrating the lesion.Increased expression of P110 was found in the epidermis of psoriatic lesions compared with the lesions in the other disorders,whereas no significant difference was noticed among the other disorders.In the case of P85 and P110 expression in the lesion-infiltrating lymphocytes infiltrating the lesion,psoriasis and squamous cell carcinoma significantly differed from the other disorders,while no difference was observed between psoriasis and squamous cell carcinoma (P＞0.05).Conclusions The high expression of P110 might be closely correlated to the hyperproliferation of keratinocytes;but filrther study is needed to clarify the relationship of increased expression of P85 and P110 to the activation and proliferation of lymphocytes in psoriatic lesions.