细菌培养快速药敏与鉴定试验探讨

目的：探讨用快速方法进行细菌鉴定与药敏试验。方法：1．快速法：对Bact／ALERT 3D培养仪显示阳性的标本进行涂片、染色、细菌计数，调整菌液浓度后，用ATB鉴定仪配套试条，直接进行鉴定与药敏试验。2．标准法。结果：对63例阳性标本用快速法与标准法进行鉴定与药敏试验进行比较。葡萄球菌，肠杆菌科细菌，非发酵菌(以铜绿假单胞菌为主)菌属符合率为95％以上，菌种符合率89％至95％以上，所有药敏试验结果符合率在95％-97．2％。结论：葡萄球菌，肠杆菌科细菌，非发酵菌快速法与标准法的鉴定、药敏试验符合率高，效果满意。

TO STUDY ON THE RAPID METHODS OF BACTERIA IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING ( AST)

Objective:To study the rapid methods of bacteria identification and antimicrobial susceptibility testing (AST) of the positive sample for Bact/ALERT system.Methods:Bacterium of positive bacteria were strained,smeared,clonied and adjusted for the concentration,Identification and AST with strips of the ATB system had been done directly.Results:In 63 positive sample results of Staphylococcus,Enterobacteriaceae and nonfermenters (most of Pseudomonas.Aeruginosa).The rate of coincidence with bacterium genus was over 95%,with bacterium species was over 89% to 95% and all of the AST coincidence was between 95% to 97.2%.Conclusions:Bacteria identification and AST with the rapid method in staphylococcus ,Enterobacteriaceae and nonfermenter has high coincidence with that of the standard methods.