c-myc反义寡核苷酸对缺氧内皮细胞条件培养液刺激的肺血管平滑肌细胞增殖的影响

目的探讨ｃ－ｍｙｃ反义寡核苷酸（ＯＤＮｓ）对缺氧内皮细胞条件培养液（ＨＥＣＣＭ）刺激的肺血管平滑肌细胞（ＳＭＣ）增殖的影响。方法制备ＨＥＣＣＭ，用其刺激肺动脉ＳＭＣ后，Ｎｏｒｔｈｅｒｎ杂交分析ｃ－ｍｙｃ基因表达，３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入试验及细胞生长计数分析ＳＭＣ增殖情况。结果ＨＥＣＣＭ显著刺激ＳＭＣ增殖及ｃ－ｍｙｃ基因表达增强，反义ＯＤＮｓ显著下调ＨＥＣＣＭ刺激的ｃ－ｍｙｃ表达，显著抑制ＨＥＣＣＭ刺激的ＳＭＣ增殖。同义ＯＤＮｓ无上述作用。结论ＨＥＣＣＭ可能通过刺激ＳＭＣ之ｃ－ｍｙｃ表达而使ＳＭＣ增殖，反义ＯＤＮｓ通过下调ｃ－ｍｙｃｍＲＮＡ表达而抑制ＨＥＣ－ＣＭ刺激的ＳＭＣ增殖。

The effect of c myc antisense oligodeoxynucleotides on proliferation of pulmonary arterial smooth muscle cells stimulated by hypoxic endothelial cells conditioned medium

Objective To study the effect of c myc antisense oligodeoxynucleotides on proliferation of pulmonary arterial smooth muscle cells (SMC) stimulated by hypoxic endothelial cells conditioned medium(HECCM). Method After pulmonary arterial SMC were stimulated by HECCM, Northern blot technique was used for detection of expression of c myc, and 3H TdR incorporation and cell growth assay for detection of proliferation of SMC. Result HECCM promoted the expression of c myc and proliferation of SMC significantly. Antisense ODNs significantly inhibited the expression of c myc and proliferation of SMC stimulated by HECCM, whereas sense ODNs didn't affect the expression of c myc and proliferation of SMC. Conclusion HECCM promote the proliferation of SMC by increasing the expression of c myc, antisense ODNs inhibits the proliferation of SMC by downregulating the expression of c myc gene.