放疗诱导宫颈癌细胞凋亡与c-myc蛋白表达

目的 :探讨在放射治疗前、中宫颈癌细胞凋亡与c myc蛋白表达的关系 .方法 :对 4 8例宫颈癌患者分别于放疗前、放疗 2 0Gy/ 2wk取活检 ,石蜡包埋 .应用原位缺口末端标记法 (TUNEL)检测细胞凋亡 ,应用免疫组化SP法对c myc蛋白进行检测 .结果 :4 8例宫颈癌患者放疗前、放疗 2 0Gy ,凋亡阳性率分别为 6 7%和 85 % ,有显著性差异 (P <0 .0 5 ) ;宫颈鳞癌患者病理分级增高 ,临床分期越晚凋亡阳性率均有降低 ,但无显著性差异 .放疗前c myc蛋白阳性表达率为 86 % ,放疗2 0Gy其表达率为 4 3% ,显著性降低 (P <0 .0 1 ) ;放疗 2 0Gy,c myc蛋白表达与凋亡有明显相关 .结论 :c myc蛋白表达与放射诱导宫颈癌细胞凋亡有关

Relationship of apoptosis and c-myc protein expression with cervical carcinoma before and during radiation therapy

AIM: To study the relationship between apoptosis and c myc protein in cervical carcinoma. METHODS: Speciments were excised from the advanced cervical squamous carcinoma of 48 patients before and during fractionated radiation therapy. The tumor irradiation dose was 20 Gy/2 weeks. TdT mediated bio dUTP nick end labeling (TUNEL) method was used to observe apoptotic cells in formalin fixed paraffin embedded tissues. c myc protein expression was immunohistochemically examined. RESULTS: The positive rate of apoptosis increased significantly from 67% to 85% after 20 Gy was administered( P <0.05). There was no significant correlation ( P >0.05) between the positive rate of apoptosis and the tumor histological differentiation and clinical stages. The positive rate of c myc decreased from 78% to 43% after 20 Gy was administered. It has a statistically significant change ( P <0.01). There was a significant association between radiation induced apoptosis and c myc expresson in 20 Gy radiation ( P <0.01). CONCLUSION: The results suggest that radiation induced apoptosis is correlated with the expression of c myc proteins of cervical carcinoma.