黑色素肿瘤抗原基因-1、3在直肠癌肝转移组织的表达

目的探索黑色素肿瘤抗原基因-1、-3（MAGE-1、MAGE-3）在直肠癌肝转移组织中的表达与直肠癌肝转移的相关性。方法采用RT-PCR法，对30例直肠癌肝转移患者的癌组织和30例直肠癌非肝转移患者的癌组织的MAGE-1、MAGE-3的表达情况进行检测，并对RT-PCR扩增产物中的目的基因片段进行DNA测序验证。结果30例直肠癌肝转移患者癌组织中，MAGE-1基因的表达阳性率为46．67％，MAGE-3基因的表达阳性率为56．67％，MAGE-1、MAGE-3都表达的阳性率为36．67％，至少表达其中一种的阳性率为66．67％；30例直肠癌非肝转移患者癌组织中，MAGE-1基因的表达阳性率为26．67％，MAGE-3基因的表达阳性率为40．00％，MAGE-1、MAGE-3都表达的阳性率为23．33％，至少表达其中一种的阳性率为43．33％。直肠癌肝转移患者癌组织中MAGE-1、MAGE-3基因表达阳性率均显著高于直肠癌非肝转移患者癌组织（P〈0．05）。结论MAGE-1，MAGE-3有望作为一种新的指标用于直肠癌的转移和预后监测。

Expression of Melanoma Antigen Gene-1,3 in Rectal Carcinoma with Hepatic Metastasis and Its Clinical Significance

Objective To detect the expression of melanoma antigen-1 gene （ MAGE-1 ）, melanoma antigen-3 gene（ MAGE-3 ） in rectal carcinoma with hepatic metastasis and explore its clinical significance. Methods The expression of MAGE-1 and MAGE-3 in central tumor tissue of rectal carcinoma with or without hepatic metastasis were detected by RT-PCR. Results Expression of MAGE-1 was positive in 14 out of 30 （46. 67% ） eases and expression of MAGE-3 was positive in 17 out of 30 （56.67%） eases in rectal carcinoma with hepatic metastasis respectively ; 11 out of 30 cases （ 36.67% ） expressed MAGE-1 and MAGE-3 simutaniously ; 20 out of 30 cases （66.67%） expressed at least one of MAGE-1 and MAGE-3; Expression of MAGE-1 was positive in 8 out of 30 （26.67%） cases and expression of MAGE-3 was positive in 12 out of 30 （40.00%） ca- ses in rectal carcinoma without hepatic metastasis respectively; 7 out of 30 eases （23.33%） expressed MAGE- 1 and MAGE-3 simutaniously ; 13 out of 30 cases （43.33%） expressed at least one of MAGE-1 and MAGE-3 in rectal carcinoma without hepatic metastasis; the positive rates of MAGErl or MAGE-3 of rectal carcinoma with hepatic metastasis were significant higher than those of rectal carcinom without hepatic metastasis （ P 〈 0. 05）. Conclusion MAGE-1 and MAGE-3 protein may be used as a target molecule of immunotherapy for rectal cancer and indexes for follow-up study of rectal carcinoma as well.