| 酶法提取杜仲中降压活性成分 |
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| 引用本文： | 曹慧,陈晓青,肖建波. 酶法提取杜仲中降压活性成分[J]. 中国生化药物杂志, 2005, 26(4): 203-205 |
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| 作者姓名： | 曹慧 陈晓青 肖建波 |
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| 作者单位： | 中南大学化学化工学院,湖南长沙410083 |
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| 基金项目： | 国家自然科学基金重大研究计划项目（No.C03050205）及湖南省科研计划项目（No.02JZ13029） |
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| 摘 要： | 目的采用酶法提取杜仲中降压活性成分。方法选用纤维素酶,通过L9(34)正交试验确定京尼平苷酸和京尼平苷最佳提取工艺条件。结果提取京尼平苷酸的最佳工艺参数:温度45℃,介质pH=5,酶解时间1h,每100g杜仲需纤维素酶0.3g,提取率达到1.58%;提取京尼平苷的最佳工艺参数为:温度45℃,介质pH=7,酶解时间0.5h,每100g杜仲需纤维素酶0.2g,提取率达到0.26%。结论与传统水浸提取工艺相比,提取率提高9.09%~14.45%。
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| 关 键 词： | 杜仲 纤维素酶 提取 正交设计 |
| 文章编号： | 1005-1678（2005）04-0203-03 |
| 收稿时间： | 2004-10-21 |
| 修稿时间： | 2005-04-26 |
Extraction of hypotensive constituents from E. ulmoides by enzymolysis |
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| CAO Hui,CHEN Xiao-qing,XIAO Jian-bo. Extraction of hypotensive constituents from E. ulmoides by enzymolysis[J]. Chinese Journal of Biochemical Pharmaceutics, 2005, 26(4): 203-205 |
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| Authors: | CAO Hui CHEN Xiao-qing XIAO Jian-bo |
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| Abstract: | PurposeThe extraction of hypotensive constituents from E.ulmoides was studied by enzymolysis.MethodsCellulase was chosen to carry out the experiment and the optimum extraction conditions were determined by orthogonal design method L_9(3~4).ResultsThe optimum extraction condition of GPA was as follows: 45℃ as extraction temperature, 5.0 as pH of solvent,1 h as extraction time and 100∶(0.3) as optimum solid-enzyme ratio. While the optium extraction condition of GPS was as follows: 45℃ as extraction temperature, 7.0 as pH of solvent, 0.5 h as extraction time and 100∶0.2 as optimum solid-enzyme ratio. Under these conditions, the extraction percentage of GPA and GPS was 1.58% and 0.26% respectively.ConclusionThe extraction percentage of GPA and GPS using above method was higher than that of water extraction. |
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| Keywords: | E. ulmoides cellulase extraction orthogonal experiment |
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