| miRNA－92a在缺血再灌注肾损伤中的表达变化 |
| |
| 引用本文： | 吴珏,刘芬,阮琼芳,娄远蕾,郭菲,杨阳,汪泱.miRNA－92a在缺血再灌注肾损伤中的表达变化[J].江西医学检验,2010(1):9-11. |
| |
| 作者姓名： | 吴珏 刘芬 阮琼芳 娄远蕾 郭菲 杨阳 汪泱 |
| |
| 作者单位： | 南昌大学泌尿外科研究所; |
| |
| 基金项目： | 国家自然科学基金(30960385) |
| |
| 摘 要： | 目的观察miRNA-92a在缺血再灌注（I／R）损伤4h、24h后小鼠肾组织的表达变化，探讨肾脏缺血损伤后血管生成的可能机制。方法采用双侧夹闭小鼠肾蒂的方法制备急性缺血再灌注肾损伤模型，术后灌注24h后收集血清和。肾脏标本，分别检测肾功能及观察肾组织病理学改变。实时定量逆转录聚合酶链反应（real—time RT-PCR）的方法检测小鼠I／R损伤4h和24h组中miRNA-92a的表达水平。结果（1）采用血清肌酐（Scr）和尿素氮（UN）评估肾功能，肾脏I／R 24h组与假手术组（sham）比较有显著性差异（P〈0．05）；HE染色观察肾组织病理改变明显，肾缺血再灌注模型成功。（2）而I／R4h和24h后，miRNA-92a的表达上调，其上调倍数分别为3．23±0．74．1．53±0．33（P〈0．05）。结论小鼠I／R损伤中miRNA-92a表达显著上调，miRNA-92a可能参与缺血再灌注肾组织损伤的血管再生的调控过程。
|
| 关 键 词： | 微小RNA 小鼠 肾脏 缺血再灌注损伤 |
Expression of miRNA-92a affected by renal ischemia/reperfusion injury in mice |
| |
| WU Jue,LIU Fen,RUAN Qiongfang,et al..Expression of miRNA-92a affected by renal ischemia/reperfusion injury in mice[J].Jiangxi Journal of Medical Laboratory Sciences,2010(1):9-11. |
| |
| Authors: | WU Jue LIU Fen RUAN Qiongfang |
| |
| Institution: | WU Jue,LIU Fen,RUAN Qiongfang,et al.Institute of Urology of Nanchang University,Nanchang 330006,China |
| |
| Abstract: | Objective To investigate the expression change of miRNA-92a in kidney ischemia/reperfusion(I/R) injury in mice.Methods Mice were subjected to a standard renal I/R to induce acute kidney injury(AKI) after 45 min of bilateral renal artery clamping.miRNA-92a expression changes used quantitative RT-PCR analysis at 4h and 24h.Results(1)Blood samples were obtained at 24 h and at the time of animal death to evaluate the degree of AKI according to serum creatinine(SCr) and urea nitrogen(UN)(P<0.05),and the renal ti... |
| |
| Keywords: | microRNAs Mouse Kidney Ischemia/Reperfusion injury |
| 本文献已被 CNKI 维普 等数据库收录！ |
