| 小鼠视网膜中雌激素受体的表达 |
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| 引用本文： | 彭婷婷,姜发纲. 小鼠视网膜中雌激素受体的表达[J]. 临床眼科杂志, 2008, 16(1): 81-84 |
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| 作者姓名： | 彭婷婷 姜发纲 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院眼科,武汉,430022;华中科技大学同济医学院附属协和医院眼科,武汉,430022 |
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| 摘 要： | 目的观察雌激素受体（ERα）在小鼠眼视网膜中的表达及其基因表达的性别和年龄差异。方法随机取正常小鼠雌雄各五只处死后立即取出眼球，用免疫组织化学技术来观察和定位小鼠眼球视网膜中的雌激素受体，RT-PCR技术检测ERαmRNA。结果小鼠眼视网膜中ERα免疫组化染色呈阳性，视网膜组织的RT-PCR检测进一步确证ERα的存在。结论小鼠眼视网膜中有雌激素受体的表达，它们的存在可能在小鼠视网膜上有重要的生理功能，在某些眼病的发生发展方面可能有重要的病理生理学意义。
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| 关 键 词： | 小鼠 视网膜 雌激素受体 |
| 收稿时间： | 2007-07-30 |
| 修稿时间： | 2007-07-30 |
The expression of estrogen receptor alpha in rat's retina |
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| PENG Ting-ting,JIANG Fa-gang. The expression of estrogen receptor alpha in rat's retina[J]. Journal of Clinical Ophthalmology, 2008, 16(1): 81-84 |
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| Authors: | PENG Ting-ting JIANG Fa-gang |
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| Affiliation: | PENG Ting-ting, JIANG Fa-gang. (Department of Ophthalmology, Union Hospital , Tongji Medical College ,Huazhong University of Science and Technology , Wuhan 430022, China) |
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| Abstract: | Objective To observe the expression of estrogen receptor alpha（ ERα） and influence of gender and age on the gene expression in rat ＇ s retina. Methods 5 female rats and 5 male rats were obtained randomly and taken out of eyeballs immediately after being killed. Immunohistochemieal technique was used to observe and localize the estrogen receptor alpha（ ERα） in rat＇ s retina. ERαmRNA was detected by RT-PCR. Results ERαimmunostaining was observed in rat＇ s retina. The RT-PCR detection of retina further identified the exist of ERα. Conclusion There axe estrogen receptor expression in rat＇ s retina. They maybe play important roles in physiological functions of retina and have important pathophysiology significance in some oculopathy ＇ s developmem . |
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| Keywords: | Rat Retina Estrogen receptor |
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