| HPLC法测定三七发酵产物中人参皂苷Rg3含量的变化 |
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| 引用本文： | 王青,袁萍,茅仁刚,王菊勇,郑展.HPLC法测定三七发酵产物中人参皂苷Rg3含量的变化[J].中医药学刊,2011(9):2063-2064. |
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| 作者姓名： | 王青 袁萍 茅仁刚 王菊勇 郑展 |
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| 作者单位： | [1]上海中医药大学附属龙华医院中医肿瘤研究所,上海200032 [2]上海师范大学生命与环境科学学院,上海200234 |
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| 基金项目： | 基金项目：上海市科委创新行动计划项目（09140903600）;上海市教委项目（10YZS1） |
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| 摘 要： | 目的：建立经真菌转化前后三七中人参皂苷R昏的含量测定方法。方法：采用HPLC法，色谱柱为PhenomexC18（250mm×4．6mm，5μm），流动相为乙腈一水，梯度洗脱；柱温30％，检测波长为203nm，流速1．0mL／min，进样量为20μL。结果：人参皂苷Rg3的回归方程：Y=35672013X～2413，r=0．99992，在0．0019～0．2488mg／mL范围内有良好的线性关系，平均回收率为99．56％，RSD=1．48％（n=8）。结论：该方法简便、快捷、准确、重复性好，可用于三七发酵产物中人参皂苷Rg3的含量测定。
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| 关 键 词： | 三七 人参皂苷Rg3 高效液相色谱法 含量测定 |
Determination of the Content Changes of Ginsenoside Rg3 in Panax Pseudo- ginseng Fermentation Product by HPLC |
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| WANG Qing,YUAN Ping,MAO Ren-gang,WANG Ju-yong,ZHENG Zhan.Determination of the Content Changes of Ginsenoside Rg3 in Panax Pseudo- ginseng Fermentation Product by HPLC[J].Study Journal of Traditional Chinese Medicine,2011(9):2063-2064. |
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| Authors: | WANG Qing YUAN Ping MAO Ren-gang WANG Ju-yong ZHENG Zhan |
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| Institution: | 1. Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 2. Shanghai Normal University, Shanghai 200234 ,China) |
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| Abstract: | Objective ： To establish a assay method of ginsenoside Rg3 in Panax pseudo - ginseng before and after the fungal transformation by HPLC. Methods： A Phenomex C18 （250mm × 4.6mm, 5 μm） column served as stationary phase and acetonitrile -water in gradient elution mode as mobile phase; The column temperature was 30℃ and the detection wavelength was 203nm, the flow rate was 1.0mL/min and the injection volume was 20μL. Results： A good linearity of ginsenoside Rg3 was in the range of 0. 0019 ~ 0.2488 mg/mL and r = 0. 99992; The average recovery rate was 99.56% and RSD = 1.48% （ n = 8）. Conclusion： This method is simple, fast, accurate, reproducible and can be used for determining the content of ginsenoside Rg3 in Panax pseudo - ginseng before and after the fungal transformation. |
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| Keywords: | Panax pseudo - ginseng Ginsenoside Rg3 HPLC Content determination |
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