RP—HPLC法测定脂降宁片中葛根素、二苯乙烯苷的含量

目的：建立高效液相色谱法测定脂降宁片中葛根素、二苯乙烯苷的含量。方法：采用kromasilC18柱（4．6mm×250mm，5μm）；流动相为甲醇（A）-水（B），梯度洗脱；流速1．0mlMmin；检测波长为250nm及320nm；枉温：30℃。结果：葛根素进样量在232—29μg范围内（r=0．9998），二苯乙烯苷进样量在220—19．5μg范围内（r=0．9997）线性关系良好，平均回收率葛根素为97．2％（RSD：0．8％），二苯乙烯苷为97．6％（RSD=0．7％）。结论：该方法操作简便，分离度高，重复性好，可同时测定脂降宁片中葛根素、二苯乙烯苷的含量。

Determination of Puerarin and 2,3,5,4 - Tetrahydroxystilbene -2 - O - 13 - D - Glucoside in Zhijiangning Tablets by RP- HPLC

Objective ： To establish an HPLC method for determination of Puerarin and 2,3,5,4 - tetrahydroxystilbe- ne-2- O- β -D -glucoside in Zhijiangning tablets. Methods ：The column was kromasil C18 （4.6 mm × 250 mm, 5 μm）. The mobile phase was A （methanol） - B （water）with gradient elution and the flow rate was 1. 0mL/min, The detection wavelength were 250nm and 320nm. The column temperature was 30℃ o RESULTS：The linear rangs of Puerarin and 2,3,5,4 - tetrahydroxystilbene - 2 - O - β - D - glucoside were 232 ~ 291xg（ r = 0. 9998 ） and 220 ~ 19.5μg（ r = 0.9997 ）. The average recoveries were 97.2% and 97.6%. Conclusion ： The method is simple, accurate and good repro- ducibility. It can be used foe the determination of Puerarin and 2,3,5,4 - tetrahydroxystilbene - 2 - O - β- D - gluco- side in Zhijiangning tablets.