| 多波长RP—HPLC测定山楂叶中芦丁、金丝桃苷及槲皮素含量 |
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| 引用本文： | 张雪莹,张杨,张建逵,韩荣春,康廷国,王冰.多波长RP—HPLC测定山楂叶中芦丁、金丝桃苷及槲皮素含量[J].中医药学刊,2011(9):1987-1989. |
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| 作者姓名： | 张雪莹 张杨 张建逵 韩荣春 康廷国 王冰 |
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| 作者单位： | [1]沈阳佰佳安医疗健康用品有限公司,辽宁沈阳110031 [2]大连华立金港药业有限公司,辽宁大连116100 [3]辽宁中医药大学,辽宁大连116600 |
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| 基金项目： | 基金项目：国家科技基础条件平台项目（2005DKA21000） |
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| 摘 要： | 目的：建立同时测定山楂叶中芦丁、金丝桃苷及槲皮素的分析方法。方法：采用多波长RP—HPLC法，色谱柱为AgilentEclipseXDB—C18柱（4．6mm×150mm，5μm）；流动相：乙腈（A）-0．025mol／L磷酸水（B），梯度洗脱依次为0—15min（18％A～18％A），15～30min（18％A-40％A）；检测波长为363nm（0—18min），371nm（18—30min）；流速：0．9mL／min；柱温：28℃。结果：芦丁、金丝桃苷及槲皮素的线性范围分别为0．3148～1．5740μg、0．4205～2．1024μg、0．0261—0．1305μg。平均加样回收率分别为100．29％、99．42％、98．86％，RSD分别为1．79％、1．46％、1．50％。结论：该方法简便、快速、准确，重现性好，可用于山楂叶中芦丁、金丝桃苷及槲皮素的同时测定。
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| 关 键 词： | 反相高效液相色谱 山楂叶 芦丁 金丝桃苷 槲皮素 |
Simultaneous Determination of Rutin, Hyperoside, Quercetin in Folium Crataegi by Multiple wavelength RP -HPLC |
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| ZHANG Xue-ying,ZHANG Yang,ZHANG Jian-kui,HAN Rong-chun,KANG Ting-guo,WANG Bing.Simultaneous Determination of Rutin, Hyperoside, Quercetin in Folium Crataegi by Multiple wavelength RP -HPLC[J].Study Journal of Traditional Chinese Medicine,2011(9):1987-1989. |
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| Authors: | ZHANG Xue-ying ZHANG Yang ZHANG Jian-kui HAN Rong-chun KANG Ting-guo WANG Bing |
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| Institution: | 1. Shenyang Bai Jia-an Health Products Co. Ltd, Shenyang 110031, Liaoning, China ; 2. Dalian Holley Kingkong Pharmaceutical Co. , Ltd. , Da｜ian 116100, Liaoning, China; 3. Liaoning University of TCM, Dalian 116600, Liaoning, China) |
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| Abstract: | AIM： To establish a method for simultaneous determination of rutin, hyperoside, quercetin in Folium Cra- taegi. Methods： A multiple wavelength RP- HPLC method was developed. The analysis was performed on an Agilent E- clipse XDB - C18column （4.6mm 150mm, 5μm）. A mixture of acetonitrile （A） - 0. 025mol/L phosphoric acid （B） with gradient elution as the mobile phase was adopted. The gradient elution program was 0 ~ 15min （18% A ~ 18% A）, 15 ~ 30min （ 18% A ~ 40% A） ; The wavelength was monitored 363nm （0 ~ 18min）, 371nm（ 18 - 30min）. The flow rate was 0.9 mL/min. The column temperature was 28 ℃. Results：The linear response ranges of rutin, hyperoside, quercetin were 0. 3148 - 1. 57401xg, 0. 4205 ~ 2. 1024μg, 0. 0261 - 0. 1305txg respectively. The average recoveries were 100.29%, 99.42%, 98.86% with RSD of 1.79%, 1.46%, 1.50% respectively. Conclusion： The method is simple and reproducible for determining rutin, hyperoside, quercetin in Folium Crataegi. |
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| Keywords: | RP - HPLC Folium Crataegi rutin hyperoside quercetin |
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