| 斑蝥酸钠逆转K562/AO2细胞多药耐药的机制初探 |
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| 引用本文： | 魏素菊,时爱春.斑蝥酸钠逆转K562/AO2细胞多药耐药的机制初探[J].实用肿瘤杂志,2007,22(5):401-404. |
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| 作者姓名： | 魏素菊 时爱春 |
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| 作者单位： | 河北医科大学第四医院肿瘤内科,河北,石家庄,050011 |
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| 摘 要： | 目的 观察斑蝥酸钠(SCA)对多药耐药的白血病细胞(K562/AO2)细胞是否有逆转作用,并初步探讨其逆转机制.方法 应用 MTT测定多柔比星(阿霉素,ADM)对K562/AO2细胞的半数抑制浓度(IC50);应用逆转录聚合酶链式反应(RT-PCR)检测K562、K562/AO2细胞表达mdr1基因水平;用流式细胞术(FCM)检测其P-gp蛋白表达的水平.结果 MTT结果显示,无毒剂量的SCA与ADM联合应用比单纯加ADM(同等剂量)对K562/AO2细胞的IC50降低了1.51倍;耐药细胞(K562/AO2)经SCA处理后mdr1基因与P-gp蛋白表达均下降(P＜0.05).结论 SCA对K562/AO2有一定的逆转作用,其机制可能与下调mdr1基因和P-gp蛋白表达有关.
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| 关 键 词： | 白血病/药物疗法 肿瘤细胞 培养的 药物耐受性 斑蝥酸钠/治疗应用 糖蛋白类 |
| 文章编号： | 1001-1692（2007）05-0401-04 |
| 修稿时间： | 2007-03-29 |
Primary study on mechanism of disodium cantharidinate reversing K562/AO2 cells MDR |
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| WEI Su-ju,SHI Ai-chun.Primary study on mechanism of disodium cantharidinate reversing K562/AO2 cells MDR[J].Journal of Practical Oncology,2007,22(5):401-404. |
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| Authors: | WEI Su-ju SHI Ai-chun |
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| Institution: | The Fourth Hospital, Heibei Medical University,Shijiazhuang, 050011, China |
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| Abstract: | Objective To investigate the functional mechanism of Chinese drug disodium cantharidinate(SCA) reversing multidrug resistance of tumor cells.Methods MTT assay was used to determine IC50of ADM acting on K562/AO2;RT-PCR assay was applied to detect mdr1 gene level;and FCM was applied to determine expressional level of P-gp.Results MTT assay showed that IC50of K562/AO2in SCA used group was reduced 1.51 times compared with that in SCA non-used group;RT-PCR indicated that mdr1 gene was reduced after SCA was used.And FCM indicated Pgp expression was reduced after SCA was used on K562/AO2.Conclusion Mechanism of Chinese drug SCA reversing multidrug resistance could be concerned with down regulation of mdr1 gene and P-gp expression level. |
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| Keywords: | leakemia/drug therapy tumar cells cultured drug tolerance disodium cantharidinate/therapeutic use glycoproteins |
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