两种甾体激素避孕药对用药妇女rRNA基因功能的影响

应用人类核仁形成区(NOR)选择性银染技术,对分别注射复方己酸孕酮注射剂和复方甲地孕酮微囊注射剂,以及正常对照妇女进行外周血淋巴细胞NOR的研究。结果注射组、自身对照组及正常对照组银染核仁形成区(Ag-NOR)的分布,在丹佛体制分组水平上呈随机分布。注射组与对照组的 Ag-NOR频率,以及自身对照组注射前后的Ag-NOR频率,均无显著差异,表明这两种药物对用药妇女外周血淋巴细胞有活性的rRNA基因数目没有影响。注射组和自身对照组注射后银染的近端着丝粒染色体联合(Ag-AA)频率,均比对照组和自身对照组注射前显著增高,表明这两种药物有增加用药妇女外周血淋巴细胞rRNA基因活性的作用。

THE EFFECT OF TWO STEROID CONTRACEPTIVES ON THE FUNCTION OF rRNA GENES OF USERS

The nucleolus organizer regions(NORs)in the peripheral blood lymphocytes inthree groups of women were studied bythe selective Ag-staining technique:(1)In-jection with hydroxyprogesterone caproatecompoind or microencapsulated megesterolacetate compound;(2) no contraceptiveused;(3) either one of the two contraceptivesused with the period before medication serv-ing as selfcontrol.The Ag-NORs in thethree groups were randomly distributed onthe level of the Denver system.The Ag-NOR frequencies among the injected group,the control group and the pre-and-postinjection showed no significant difference.These results indicated that two steroid con-traceptives had no effect on the numbersof the activated rRNA genes.The Ag-AA(Ag - acrocentric - association) frequencies ofthe injected group and the post-injectionwere significantly higher than the controlgroup and the pre-injection.The resultsuggests that the two steroid contracep-tives may have an effect on the increase ofthe activation of the rRNA genes inlymphocytes.