未折叠蛋白反应参与小鼠急性肾缺血/再灌注损伤

目的 探讨未折叠蛋白反应(UPR)在小鼠急性肾缺血/再灌注损伤中的作用.方法 将C5786小鼠分为正常、假手术组和0、4、12、24 h手术组(分别于术后0、4、12和24 h处死小鼠),留取各组小鼠的左侧肾脏.苏木精一伊红(H-E)、过碘酸雪夫反应(PAS)和脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)染色观察各组肾脏组织学变化,Western印迹法和实时聚合酶链反应(PCR)检测葡萄糖调节蛋白78(GRP78)和caspasel2在各组小鼠肾组织中的表达.结果 H-E、PAS染色可见24 h手术组的肾小管上皮细胞坏死明显,病变最重的部位为外髓外带近端小管,肾小管坏死评分为(2.930 0±0.131 1)分,显著高于其他组(P值均<0.01).TUNEL染色可见24 h手术组的肾小管上皮细胞凋亡指数为(36.17±7.53)%,显著高于其他各组(P值均<0.01).Western印迹法.结果 显示,12 h手术组的GRP78和caspase12蛋白水平显著高于其他各组(P值均<0.01).实时PCR.结果 显示,4 h手术组的GRP78和caspasel2 mRNA水平显著高于其他各组(P值分别<0.01、0.05).结论 在夹闭左侧肾动脉致急性肾缺血/再灌注损伤模型中,小鼠左侧肾脏组织学损伤显示再灌注后24 h最严重.GRP78和caspasel2在再灌注后其mRNA和蛋白表达水平明显升高或激活,其变化早于组织学改变.提示UPR可能在急性肾缺血/再灌注损伤中发挥重要作用.

Unfolded protein response involves in acute renal ischemia/reperfusion injury in mice

Objective To explore the role of unfolded protein response(UPR) in acute renal ischemia/reperfusion injury(ARIRI) in mice.Methods C57B6 mice were divided into the normal group,sham operation group and the surgery group;the surgery group was further divided into 0 h,4 h,12 h,and 24 h subgroups according to the time they were put into death.The left kidneys of the mice were harvested in each group.H-E,PAS staining and TUNEL staining were employed to observe the histological changes of the kidney.Western-blot ...