肝癌微血管密度、微血管面积和Piezo1的表达与微血管侵犯的相关性

目的:探讨肝癌组织中微血管密度(microvascular density,MVD)、微血管面积(microvascular area,MVA)以及Piezo1的表达水平预测肝癌微血管侵犯(microvascular invasion,MVI)的临床价值.方法:应用免疫组织化学方法检测38例病理证实为肝癌患者的肝癌组织的CD34以及Piezo1的表达情况,计算基于CD34染色的MVD和MVA,分析MVI与MVD,MVA以及Piezo1因子的表达水平的相关性.结果:38例肝癌中,13例有微血管侵犯,定义MVI(+)组,25例无微血管侵犯,定义MVI(?)组.MVI(+)组的MVA及MVD均高于MVI(?)组,两组间差异有统计学意义(P=0.007,P=0.011).MVD和MVA联合预测MVI的敏感性和特异性为100％和64％,较单一指标效能高.Piezo1在肝癌MVI(+)组阳性率高于MVI(?)组,两组间差异有统计学意义(P=0.032).结论:MVD,MVA以及Piezo1的表达水平均与肝癌MVI具有一定的相关性,可以作为辅助诊断微血管侵犯的指标,Piezo1可以作为潜在的限制MVI的治疗靶点.

Correlation between microvascular density,microvascular area,Piezo1 expression and microvascular invasion in hepatocellular carcinoma

Objective: To investigate the clinical value of microvascular density (MVD), microvascular area (MVA) and the expression of Piezo1 in predicting microvascular invasion (MVI) of hepatocellular carcinoma (HCC). Methods: Immunohistochemical method was applied to detect the expression of CD34 and Piezo1 of 38 pathologically confirmed HCC for 38 patients, MVD and MVA were measured based on CD34 staining. The correlations between the expression level of Piezo1, MVD, MVA and MVI were analyzed. Results: Thirteen in 38 cases were presented with MVI, defined as MVI (+) group, 25 in 38 cases were absence of MVI, defined as MVI (?) group. The MVA and MVD were significantly higher in MVI (+) group (P=0.007, P=0.011; respectively) than in MVI (?) group. MVD combined with MVA achieved a sensitivity of 100％ and a specificity of 64％ in predicting MVI of HCC, which was higher than single index. The expression level of Piezo1 was significantly higher in the MVI (+) group than in the MVI (?) group (P=0.032). Conclusion: MVD, MVA and the expression level of Piezo1 are significantly correlated with MVI which can be used as surrogate markers of MVI, Piezo1 may be a novel therapy target for restraining MVI.