Ento-Ⅰ涂膜剂经皮给药对小鼠耐缺氧和抗心肌缺血作用初探

目的 观察Ento-Ⅰ涂膜剂的耐缺氧及抗心肌缺血作用.方法 采用检测小鼠常压耐缺氧时间、小鼠缺血缺氧喘息时间和张口呼吸次数以及小鼠亚硝酸钠中毒存活时间,探索Ento-Ⅰ涂膜剂的耐缺氧作用.实验分组为生理盐水组、涂膜剂基质组、阿司匹林组和Ento-Ⅰ涂膜剂组(9.125、18.25、37.5 mg/kg);通过皮下注射异丙肾上腺素致小鼠心肌缺血模型初步评价Ento-Ⅰ涂膜剂的抗心肌缺血作用,实验分组为生理盐水组、涂膜剂基质组、复方丹参滴丸组和Ento-Ⅰ涂膜剂组(5、10、20 mg/kg).实验中各组给药方式如下:生理盐水组、阿司匹林和复方丹参滴丸组为灌胃,涂膜剂基质组及Ento-Ⅰ涂膜剂组为涂抹于太阳穴两侧.结果 4个实验中涂膜剂基质组与生理盐水组比较,所有指标均无统计学差异.与生理盐水组比较,Ento-Ⅰ涂膜剂中剂量组(18.25 mg/kg)小鼠常压耐缺氧时间(50.87±11.90)min、亚硝酸钠中毒后存活时间(14.15±4.61)min和断头后喘息时间(27.90±4.79)s均显著延长(P<0.01).抗心肌缺血实验中,与生理盐水组比较,Ento-Ⅰ涂膜剂高剂量组(20 mg/kg)小鼠缺血心肌超氧化物歧化酶活性显著升高(P<0.01),丙二醛含量显著降低(P<0.01);各组小鼠缺血心肌病理切片结果表明Ento-Ⅰ涂膜剂可明显减轻缺血心肌损伤,增强心肌抗缺血作用.结论 Ento-Ⅰ涂膜剂可有效提高小鼠耐缺氧和抗缺血的能力.

Effects of Ento-Ⅰplastic on anti-anoxia and myocardial ischemia in mice:a preliminary study

Objective To investigate the effects of Ento-Ⅰplastic on anoxia and myocardial ischemia. Methods The effect of Ento-Ⅰplastic on anoxia in mice was evaluated by testing mice's anti-anoxia time under ordinary pressure,mice's gasp time and the number of mouth breathing under ischemia-anoxia,and mice's survival time after sodium nitrite poisoning. Animals were divided into normal saline(NS)group,matrix group,aspirin(ASP)group and Ento-Ⅰplastic(9.125,18.25 and 37.5 mg/kg)groups. The ef?fect of Ento-Ⅰon myocardial ischemia in mice was preliminarily evaluated using the animal model of acute myocardial ischemia in?duced by the subcutaneous injection of isoprenaline(the animals were divided into NS group,matrix group,compound danshen drop?ping pill(DSP)group and Ento-Ⅰplastic(5,10 and 20 mg/kg )groups. In these experiments,NS group,ASP group and DSP group were administered intragastrically,while the matrix group and Ento-Ⅰplastic groups were administered by daubing on both sides of the temple. Results There was no significant difference in all indexes between the NS group and the matrix group in all four experi?ments. Compared with NS,the middle dosage of Ento-Ⅰplastic(18.25 mg/kg)could obviously prolong the anoxia tolerance time to (50.87 ± 11.90)min under ordinary pressure,survival time to(14.15 ± 4.61)min after sodium nitrite poisoning and the gasp time to(27.90 ± 4.79)s after decapitation,with the statistically significant difference(P<0.01). In anti-myocardial ischemia experiment, both the myocardial superoxide dismutase(SOD)activity and malondialdehyde(MDA)content were significantly decreased in the high dosage Ento-Ⅰ(20 mg/kg)and DSP groups than in the NS group(P<0.01). The pathological examination of the ischemic of myocardium itself showed that Ento-Ⅰplastic could significantly reduce ischemic myocardial injury and enhance the anti-ischemic ef?fect of myocardium itself. Conclusion The Ento-Ⅰplastic could obviously strengthen the anti-anoxic and anti-ischemic ability of mice.