氯化镉对小鼠腹腔巨噬细胞功能的影响

目的观察镉对巨噬细胞功能的影响。方法以０．３、０．６、１．２ｍｇ／ｋｇ体重ＣｄＣｌ２连续给小鼠灌胃１４天后进行巨噬细胞吞噬功能、产生ＮＯ的能力及产生Ｈ２Ｏ２等多个指标的检测。结果在各剂量ＣｄＣｌ２作用下，小鼠腹腔灌洗液细胞数减少，与对照组比差异有显著性。巨噬细胞吞噬功能受到明显抑制，抑制率分别为２１．８％、３９．９％、４４．４％，且呈现剂量反应关系（ｒ＝－０．９０３１）。巨噬细胞产生ＮＯ的能力也受到明显抑制，对照组为７６．１０±１４．２２μｍｏｌ／Ｌ，其于各组分别为６１．９０±２７．８３、３６６７±２３．８６、４６．６０±１３．３３μｍｏｌ／Ｌ。巨噬细胞产生的Ｏ－２在三种剂量下都出现明显抑制，呈现剂量反应关系（ｒ＝０．９７９０）。巨噬细胞产生Ｈ２Ｏ２的能力仅在１．２ｍｇ／ｋｇ剂量时出现明显抑制，与对照组比差异有显著性，但Ｈ２Ｏ２的产生与ＣｄＣｌ２剂量亦存在剂量反应关系（ｒ＝－０．９８４７）。结论ＣｄＣｌ２在０３、０．６、１．２ｍｇ／ｋｇ剂量下对巨噬细胞吞噬功能，ＮＯ、Ｏ－２、Ｈ２Ｏ２等生物分子的产生均有明显抑制。因此认为ＣｄＣｌ２可直接影响巨噬细胞的抗原递呈及非特异性防御功能

Effects of Cadmium Chloride on the Function of Peritoneal Macrophage in Mice

OBJECTIVE: To study the effects of cadmium on macrophage function. METHODS: Many indicators, such as phagocytic function, abilities to produce nitrous oxide and hydrogen peroxide, in mice were determined 14 days after they were administered continuously with 0.3, 0.6 and 1.2 mg/kg cadmiuCell count decreased in the mouse peritoneal lavage with the effects of cadmium chloride, with a significant difference, as compared with the control group. Phagocytic function of macrophage was inhibited significantly, with inhibition rates of 21.8%, 39.9% and 44.4%, respectively for various doses of cadmium, in a dose-response pattern. The ability of microphage to produce nitrous oxide was significantly inhibited, also, with 61.90 +/- 27.83, 36.67 +/- 23.86, 46.60 +/- 13.33 mumol/L, respectively, as compared with 76.10 +/- 14.22 mumol/L in the control group. The ability of microphage to produce O2.- was obviously inhibited by three different doses of cadmium, showing a dose-response relationship (r = 0.9790). The ability of microphage to produce hydrogen peroxide was inhibited only at a dose of 1.2 mg/kg of cadmium, with a significant difference as compared with the control group. There was a dose-response relationship between cadmium and production of hydrogen peroxide, with a coefficient of regression (r) of -0.9847. CONCLUSION: Cadmium chloride, at the doses of 0.3, 0.6 and 1.2 mg/kg, could inhibit the phagocytic function of microphage, and the production of biological molecules, such as nitrous oxide, O2.- and hydrogen peroxide. It indicated that cadmium chloride could directly affect the transmission of antigen and non-specific defence function of macrophage.