| 厚朴超临界提取物大鼠体内外化学成分的UPLC/Q-TOF-MS分析 |
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| 引用本文： | 芮雯,郭晓玲,冯毅凡,卢银蕊,姜苗苗,石忠峰. 厚朴超临界提取物大鼠体内外化学成分的UPLC/Q-TOF-MS分析[J]. 中药新药与临床药理, 2011, 22(6) |
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| 作者姓名： | 芮雯 郭晓玲 冯毅凡 卢银蕊 姜苗苗 石忠峰 |
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| 作者单位： | 广东药学院中心实验室,广州,510006 |
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| 基金项目： | 国家自然科学基金资助项目"基于COCKTAIL探针药物法和UPLC-MS/MS的温肾咳喘片药物动力学研究" |
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| 摘 要： | 目的 采用超高效液相色谱与串联四级杆飞行时间质谱仪联用技术( UPLC/Q-TOF-MS)对厚朴超临界萃取( SFE)提取物进行大鼠体内外化学成分分析.方法 用ACQUITY UPLC BEH C18色谱柱,以水(A)/乙腈(B)梯度洗脱,使用ESI离子源,负离子模式下采集数据.初步分析提取物的化学成分、给药后吸收入血成分及其代谢情况.结果 厚朴SFE提取物中检测到和厚朴酚、厚朴酚、龙脑基厚朴酚等5个化学成分,其中厚朴酚原型成分吸收入血,并初步鉴定其3个代谢物.结论 厚朴酚是厚朴SFE提取物在体内的直接作用物质,结合其代谢产物分析,有助于阐明厚朴SFE提取物的体内药效物质基础.
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| 关 键 词： | 厚朴 超临界萃取 超高效液相色谱与串联四级杆飞行时间质谱仪联用技术 |
Constituents Analysis of Cortex Magnoliae Officinalis Supercritical Fluid Extraction by UPLC/Q-TOF-MS in vitro and in vivo |
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| RUI Wen,GUO Xiaoling,FENG Yifan,LU Yinrui,JIANG Miaomiao,SHI Zhongfeng. Constituents Analysis of Cortex Magnoliae Officinalis Supercritical Fluid Extraction by UPLC/Q-TOF-MS in vitro and in vivo[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2011, 22(6) |
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| Authors: | RUI Wen GUO Xiaoling FENG Yifan LU Yinrui JIANG Miaomiao SHI Zhongfeng |
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| Affiliation: | RUI Wen,GUO Xiaoling,FENG Yifan,LU Yinrui,JIANG Miaomiao,SHI Zhongfeng(Central Laboratory,Guangdong Pharmaceutical College,Guangzhou 510006,China) |
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| Abstract: | Objective To analyze the consitituents of Cortex Magnoliae Officinalis supercritical fluid extraction(SFE) in vitro and in vivo by using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC/Q-TOF-MS).Methods The chromatographic separation was performed on a ACQUITY UPLC BEH C18 column with a gradient elution of water(A)-acetonitrile(B).The mass spectrometer equipped with electrospray ionization source was used as the detector and was operated under the negative ion m... |
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| Keywords: | Cortex Magnoliae Officinalis Supercritical fluid extraction UPLC/Q-TOFMS |
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