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| 六味地黄汤相关成分对蛋白酪氨酸磷酸酶1B的影响 | |
| 引用本文： | 刘继平,兰洲,傅强,罗建光,马世平,孔令义.六味地黄汤相关成分对蛋白酪氨酸磷酸酶1B的影响[J].中药新药与临床药理,2011(5). |
| 作者姓名： | 刘继平 兰洲 傅强 罗建光 马世平 孔令义 |
| 作者单位： | 1. 中国药科大学中药药理教研室,南京210009;陕西中医学院药理教研室,咸阳712046 2. 中国药科大学中药药理教研室,南京,210009 3. 中国药科大学天然药化教研室,南京,210009 |
| 基金项目： | “重大新药创制”科技重大专项: |
| 摘 要： | 目的 研究六味地黄汤中芍药苷、没食子酸、丹皮酚、梓醇、马钱苷、莫诺苷、桃叶珊瑚苷、23-乙酰泽泻内酯醇B、薯蓣皂苷元9种重要单体成分对蛋白酪氨酸磷酸酶1B (PTP-1B)的影响,以筛选PTP-1B的有效抑制剂.方法PTP Assay Kit 1(R-R-L-I-E-D-A-E-pY-A-A-R-G)中孔雀绿磷酸检测法.结果所有单体均有不同程度的抑制PTP-1B的作用,其中莫诺苷、马钱苷、丹皮酚、23-乙酰泽泻内酯醇B、芍药苷、桃叶珊瑚苷的作用较明显,与对照组比较有显著性差异(P＜0.05,P＜ 0.01).结论莫诺苷、马钱苷、丹皮酚、23-乙酰泽泻内酯醇B、芍药苷、桃叶珊瑚苷等成分可明显抑制PTP-1B,这可能是六味地黄汤降糖、降脂机制之一. |
| 关 键 词： | 六味地黄汤 蛋白酪氨酸磷酸酶1B 孔雀绿磷酸检测法 |
Effect of Related Components in Liuwei Dihuang Decoction on Protein Tyrosine Phosphatase-1B |
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| LIU Jiping,LAN Zhou,FU Qiang,LUO Jianguang,MA Shiping,KONG Lingyi.Effect of Related Components in Liuwei Dihuang Decoction on Protein Tyrosine Phosphatase-1B[J].Traditional Chinese Drug Research & Clinical Pharmacology,2011(5). | |
| Authors: | LIU Jiping LAN Zhou FU Qiang LUO Jianguang MA Shiping KONG Lingyi |
| Institution: | LIU Jiping1,2,LAN Zhou1,FU Qiang1,LUO Jianguang3,MA Shiping1,KONG Lingyi 3(1.Department of Pharmacology of Chinese Materia Medica,China Pharmaceutical University,Nanjing 210009,China,2.Department of Pharmacology,Shanxi College of Chinese Medicine,Xianyang 712046,3.Department of Natural Pharmaceutical Chemistry,China) |
| Abstract: | Objective Effects of 9 monomer components(paeoniflorin,gallic acid,paeonol,catalpol,loganin,morroniside,aucubin,23-acetyl-alisma triptolide B and diosgenin)in Liuwei Dihuang Decoction on protein tyrosine phosphatase-1B(PTP-1B) were investigated.Methods Malachite green phosphatase assay for PTP Assay Kit 1(R-R-L-I-E-D-A-E-pY-A-A-R-G) was used.Results Paeoniflorin,paeonol,loganin,morroniside,aucubin and 23-acetyl-alisma triptolide B at different concentrations significantly inhibited the activity of PTP-1B co... |
| Keywords: | Liuwei Dihuang Decoction Protein tyrosine phosphatase-1B Malachite green phosphatase assay |
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