| 不同产地粉葛的指纹图谱及PCA模式识别分析研究 |
| |
| 引用本文： | 王黎,高苏亚,李华. 不同产地粉葛的指纹图谱及PCA模式识别分析研究[J]. 中药新药与临床药理, 2011, 22(4) |
| |
| 作者姓名： | 王黎 高苏亚 李华 |
| |
| 作者单位： | 1. 西安医学院药学院,西安,710021
2. 西北大学分析科学研究所,西安,710069 |
| |
| 基金项目： | 国家自然科学基金资助项目 |
| |
| 摘 要： | 目的 建立简单可行的粉葛药材质量评价及聚类分析新方法。方法 采用色谱柱为Kromasil5-ODS C1s柱(150 mm×4.6 mm，5μm)，流动相为甲醇+乙腈(70：30)[B]和水[A]，(0.01至50 min，B相由20％升为55％)，流速为0.6 mL/min,检测波长为268 nm的色谱条件，进行HPLC指纹图谱及PCA模式识别分析。结果 有效成分葛根素在6.25～62.50 μg/mL、大豆苷元在0.62～6.15 μg/mL浓度范围内线性关系良好，平均加样回收率分别达101.29 ％(RSD 1.86％)、99.42 ％(RSD 2.45％)；同时通过PCA模式识别分析法对不同产地的粉葛药材进行了较好的聚类分析及质量评价。结论 HPLC指纹图谱及PCA模式识别分析法可以为粉葛药材的质量鉴别与质量控制提供可靠的依据。
|
| 关 键 词： | 粉葛 HPLC指纹图谱 PCA模式识别分析 |
Fingerprints and Principal Component Analysis for Pattern Recognition of Pueraria Thamsonii from Different Habitats |
| |
| WANG Li,GAO Suya,LI Hua. Fingerprints and Principal Component Analysis for Pattern Recognition of Pueraria Thamsonii from Different Habitats[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2011, 22(4) |
| |
| Authors: | WANG Li GAO Suya LI Hua |
| |
| Affiliation: | WANG Li 1,GAO Suya 1,LI Hua 2 (1.Pharmacy Department of Xi'an Medical University,Xi'an 710021,China,2.Institute of Analytical Science of Northwest University,Xi'an 710069,China) |
| |
| Abstract: | Objective To develop a new quality evaluation method and cluster analysis for Pueraria Thamsonii from different habitats.Methods The HPLC fingerprints and principal component analysis(PCA)for pattern recognition of Pueraria Thamsonii were established by series of experiments.Results The quality evaluation of Pueraria Thamsonii samples from various producing areas was achieved by PCA.Conclusion The established HPLC fingerprints and PCA for pattern recognition can be used for quality evaluation and quality co... |
| |
| Keywords: | Pueraria Thamsonii HPLC fingerprints Principal component analysis for pattern recognition |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
