高效液相色谱-蒸发光检测法测定八宝颗粒中黄芪甲苷含量

目的建立测定八宝颗粒中黄芪甲苷含量的高效液相色谱-蒸发光检测法。方法色谱柱为Kromasil100AC18柱（250mm×4.6mm,5μm）,流动相为乙腈-水（30∶70）,流速1mL/min,漂移管温度105℃,载气流速2.8L/min。结果黄芪甲苷进样量在0.1026～1.026μg范围内与峰面积线性关系良好（r=0.9994）,平均回收率为94.60%,RSD为2.71%（n=6）。结论所用方法灵敏、简便、准确,可用作八宝颗粒的质量控制。

Determination of Astragaloside in Babao Granules by HPLC-ELSD

Objective To establish a HPLC-ELSD determination method of astragaloside in Babao Granules.Methods The separation was performed on the Kromasil 100A C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was acetonitrile-water（30 ∶70）,the flow rate was 1 mL/min,the evaporative light scattering detector as the detector,the temperature of the drift tube was set at 105 ℃,the flow rate of gas was set at 2.8 L/min.Results The linear range of astragaloside was 0.102 6-1.026 μg（r=0.999 4）,the average recovery rate was 94.60%,RSD was 2.71%（n=6）.Conclusion The method is accurate,precise,repeatable and suitable for the quality control of astragaloside in Babao Granules.